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02954nam a22003135i 4500 |
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000287536 |
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20201012105958.0 |
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cr nn 008mamaa |
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190130s2019 xxu| s |||| 0|eng d |
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|a 9781493990429
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| 024 |
7 |
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|a 10.1007/978-1-4939-9042-9
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Plant MicroRNAs :
|b Methods and Protocols /
|c edited by Stefan de Folter.
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| 250 |
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|a 1st ed. 2019.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2019.
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| 300 |
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|a XII, 363 p. 70 illus., 48 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1932
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| 505 |
0 |
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|a An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants -- The Plant MicroRNA Pathway: The Production and Action Stages -- Plant miRNA Conservation and Evolution -- Bioinformatic Analysis of Small RNA Sequencing Libraries -- An Overview of miRNA and miRNA Target Analysis Tools -- miRLocator: A Python Implementation and Web Server for Predicting miRNAs from Pre-miRNA Sequences -- Prediction of Plant miRNA Targets -- Isolation and Detection Methods of Plant miRNAs -- Northern Blot Analysis of MicroRNAs and Other Small RNAs in Plants -- miRNA Detection by Stem-Loop RT-qPCR in Studying MicroRNA Biogenesis and MicroRNA Responsiveness to Abiotic Stresses -- Detection of miRNAs by Tissue Printing and Dot Blot Hybridization -- In Situ Localization of Small RNAs in Plants -- Expression Pattern of Plant miRNAs by Classical Transcriptional Fusion Constructs -- A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy -- Degradome Sequencing in Plants -- Functional Analysis of Root MicroRNAs by a Constitutive Overexpression Approach in a Composite Plant System -- The Use of MicroRNA Decoy Technologies to Inhibit miRNA Function in Arabidopsis -- Mimicry Technology: A Versatile Tool for Small RNA Suppression -- Design and High-Throughput Generation of Artificial Small RNA Constructs for Plants -- Detection of MicroRNA Processing Intermediates through RNA Ligation Approaches -- Analysis of Methylation Status of Plant MicroRNAs -- Detection of Slicer Activity by Immunopurified Plant ARGONAUTE1 -- The Isolation of Total and Membrane-Bound Polysomes from Arabidopsis and the Detection of Their Associated AGO1 and sRNAs -- Immunolocalization to Study ARGONAUTE Proteins in Developing Ovules of the Brassicaceae -- Detection of Histone Modifications Associated with miRNAs.
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| 650 |
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0 |
|a Plant genetics.
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| 650 |
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0 |
|a Gene expression.
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| 650 |
1 |
4 |
|a Plant Genetics and Genomics.
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| 650 |
2 |
4 |
|a Gene Expression.
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| 700 |
1 |
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|a de Folter, Stefan.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|