Plant MicroRNAs : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edition: | 1st ed. 2019. |
| Series: | Methods in Molecular Biology,
1932 |
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Table of Contents:
- An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants
- The Plant MicroRNA Pathway: The Production and Action Stages
- Plant miRNA Conservation and Evolution
- Bioinformatic Analysis of Small RNA Sequencing Libraries
- An Overview of miRNA and miRNA Target Analysis Tools
- miRLocator: A Python Implementation and Web Server for Predicting miRNAs from Pre-miRNA Sequences
- Prediction of Plant miRNA Targets
- Isolation and Detection Methods of Plant miRNAs
- Northern Blot Analysis of MicroRNAs and Other Small RNAs in Plants
- miRNA Detection by Stem-Loop RT-qPCR in Studying MicroRNA Biogenesis and MicroRNA Responsiveness to Abiotic Stresses
- Detection of miRNAs by Tissue Printing and Dot Blot Hybridization
- In Situ Localization of Small RNAs in Plants
- Expression Pattern of Plant miRNAs by Classical Transcriptional Fusion Constructs
- A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy
- Degradome Sequencing in Plants
- Functional Analysis of Root MicroRNAs by a Constitutive Overexpression Approach in a Composite Plant System
- The Use of MicroRNA Decoy Technologies to Inhibit miRNA Function in Arabidopsis
- Mimicry Technology: A Versatile Tool for Small RNA Suppression
- Design and High-Throughput Generation of Artificial Small RNA Constructs for Plants
- Detection of MicroRNA Processing Intermediates through RNA Ligation Approaches
- Analysis of Methylation Status of Plant MicroRNAs
- Detection of Slicer Activity by Immunopurified Plant ARGONAUTE1
- The Isolation of Total and Membrane-Bound Polysomes from Arabidopsis and the Detection of Their Associated AGO1 and sRNAs
- Immunolocalization to Study ARGONAUTE Proteins in Developing Ovules of the Brassicaceae
- Detection of Histone Modifications Associated with miRNAs.