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02662nam a22002895i 4500 |
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cr nn 008mamaa |
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180921s2018 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493987306
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| 024 |
7 |
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|a 10.1007/978-1-4939-8730-6
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Recombinant Protein Expression in Mammalian Cells :
|b Methods and Protocols /
|c edited by David L. Hacker.
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| 250 |
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|a 1st ed. 2018.
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| 260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2018.
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| 300 |
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|a XI, 311 p. 76 illus., 68 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1850
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| 505 |
0 |
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|a Transient Gene Expression in Suspension HEK293-EBNA1 Cells -- Transient Expression of Recombinant Membrane-eGFP Fusion Proteins in HEK293 Cells -- PEI-Mediated Transient Gene Expression in CHO Cells -- Stable Expression by Lentiviral Transduction of Cells -- Inducible Protein Production in 293 Cells using the piggyBac Transposon System -- Recombinant CHO Cell Pool Generation using piggyBac Transposon System -- Genome Engineering of Hybridomas to Generate Stable Cell Lines for Antibody Expression -- Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor -- CHO and HEK293 Cell Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors -- Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary Cells in Suspension -- Continuous and Integrated Expression and Purification of Recombinant Antibodies -- High Throughput Transfection of HEK293 Cells for Transient Protein Production -- Microfluidic Transfection for High-Throughput Mammalian Protein Expression -- Genome Wide High Throughput RNAi Screening for Identification of Genes Involved in Protein Production -- Targeting miRNAs with CRISPR/Cas9 to Improve Recombinant Protein Production of CHO Cells -- Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cells -- Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV) -- Considerations in the Use of Codon Optimization for Recombinant Protein Expression -- Versatile Cell-free Protein Synthesis Systems based on Chinese Hamster Ovary Cells.
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| 650 |
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0 |
|a Proteins .
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| 650 |
1 |
4 |
|a Protein Science.
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| 700 |
1 |
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|a Hacker, David L.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|