Recombinant Protein Expression in Mammalian Cells : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2018.
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| Edition: | 1st ed. 2018. |
| Series: | Methods in Molecular Biology,
1850 |
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Table of Contents:
- Transient Gene Expression in Suspension HEK293-EBNA1 Cells
- Transient Expression of Recombinant Membrane-eGFP Fusion Proteins in HEK293 Cells
- PEI-Mediated Transient Gene Expression in CHO Cells
- Stable Expression by Lentiviral Transduction of Cells
- Inducible Protein Production in 293 Cells using the piggyBac Transposon System
- Recombinant CHO Cell Pool Generation using piggyBac Transposon System
- Genome Engineering of Hybridomas to Generate Stable Cell Lines for Antibody Expression
- Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor
- CHO and HEK293 Cell Cultivation and Transfection in Single-Use Orbitally Shaken Bioreactors
- Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary Cells in Suspension
- Continuous and Integrated Expression and Purification of Recombinant Antibodies
- High Throughput Transfection of HEK293 Cells for Transient Protein Production
- Microfluidic Transfection for High-Throughput Mammalian Protein Expression
- Genome Wide High Throughput RNAi Screening for Identification of Genes Involved in Protein Production
- Targeting miRNAs with CRISPR/Cas9 to Improve Recombinant Protein Production of CHO Cells
- Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cells
- Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV)
- Considerations in the Use of Codon Optimization for Recombinant Protein Expression
- Versatile Cell-free Protein Synthesis Systems based on Chinese Hamster Ovary Cells.