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03360nam a22003135i 4500 |
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20210524160539.0 |
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cr nn 008mamaa |
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140128s2014 xxu| s |||| 0|eng d |
| 020 |
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|a 9781627037914
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| 024 |
7 |
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|a 10.1007/978-1-62703-791-4
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Ras Signaling :
|b Methods and Protocols /
|c edited by Lorenza Trabalzini, Saverio Francesco Retta.
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| 250 |
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|a 1st ed. 2014.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2014.
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| 300 |
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|a XII, 425 p. 70 illus., 39 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1120
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| 505 |
0 |
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|a The Ras Superfamily of Small GTPases: The Unlocked Secrets -- A Novel Method for the Production of Fully-Modified K-Ras 4B -- Metabolic Labeling of Ras with Tritiated Palmitate to Monitor Palmitoylation and Depalmitoylation -- Ubiquitination of Rac1 by Inhibitors of Apoptosis (IAPs) -- Ras GTPases are Both Regulators and Effectors of Redox Agents -- Biophysical and Proteomic Characterization Strategies for Cysteine Modifications in Ras GTPases -- Use of the Yeast Two-Hybrid Technology to Isolate Molecular Interactions of Ras GTPases -- Screening for MAPK Modulators Using an In-Cell Western Assay -- Behavioral Methods for the Study of the Ras-ERK Pathway in Memory Formation and Consolidation: Passive Avoidance and Novel Object Recognition Tests -- Functional Phosphoproteomics of Oncogenic KRAS Signaling -- Pull-Down Assay for Analysis of Integrin-Mediated Activation of Rap Proteins in Adherent Platelets -- Combined Pulldown and Time-Lapse Microscopy Studies for Determining the Role of Rap1 in the Crosstalk Between Integrins and Cadherins -- Fluorescence Microscopy Study of Rap1 Subcellular Localization -- An In Vitro System to Evaluate the Scaffold Function of the RalA Effector Protein RalBP1 -- Analysis of the Rit Subfamily GTPase-Mediated Signaling and Neuronal Differentiation and Survival -- Immunofluorescence Methods in Studies of the GTPase RAN and Its Effectors in Interphase and in Mitotic Cells -- High Resolution Scanning Electron Microscopy for the Imaging of Nuclear Pore Complexes and Ran-Mediated Transport -- Effector Recruitment Method to Study Spatially Regulated Activation of Ras and Rho GTPases -- Real-Time Visualization and Quantification of Native Ras Activation in Single Living Cells -- Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis -- Analyzing the Roles of Rho GTPases in Cancer Cell Migration with a Live Cell Imaging 3D-Morphology-Based Assay -- Analysis of Rho-GTPase-Induced Localization of Nanoscale Adhesions Using Fluorescence Nanoscopy -- Yeast as a Model for Ras Signaling -- Methods to Study the Ras2 Protein Activation State and the Subcellular Localization of Ras-GTP in Saccharomyces cerevisiae -- RAS Proteins Signaling in the Early Metazoan Dictyostelium discoideum.
|
| 650 |
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0 |
|a Cell biology.
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| 650 |
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0 |
|a Proteomics.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Proteomics.
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| 700 |
1 |
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|a Trabalzini, Lorenza.
|e editor.
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| 700 |
1 |
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|a Retta, Saverio Francesco.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|