Ras Signaling : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Trabalzini, Lorenza. (Editor ), Retta, Saverio Francesco. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1120
Materias:
Tabla de Contenidos:
  • The Ras Superfamily of Small GTPases: The Unlocked Secrets
  • A Novel Method for the Production of Fully-Modified K-Ras 4B
  • Metabolic Labeling of Ras with Tritiated Palmitate to Monitor Palmitoylation and Depalmitoylation
  • Ubiquitination of Rac1 by Inhibitors of Apoptosis (IAPs)
  • Ras GTPases are Both Regulators and Effectors of Redox Agents
  • Biophysical and Proteomic Characterization Strategies for Cysteine Modifications in Ras GTPases
  • Use of the Yeast Two-Hybrid Technology to Isolate Molecular Interactions of Ras GTPases
  • Screening for MAPK Modulators Using an In-Cell Western Assay
  • Behavioral Methods for the Study of the Ras-ERK Pathway in Memory Formation and Consolidation: Passive Avoidance and Novel Object Recognition Tests
  • Functional Phosphoproteomics of Oncogenic KRAS Signaling
  • Pull-Down Assay for Analysis of Integrin-Mediated Activation of Rap Proteins in Adherent Platelets
  • Combined Pulldown and Time-Lapse Microscopy Studies for Determining the Role of Rap1 in the Crosstalk Between Integrins and Cadherins
  • Fluorescence Microscopy Study of Rap1 Subcellular Localization
  • An In Vitro System to Evaluate the Scaffold Function of the RalA Effector Protein RalBP1
  • Analysis of the Rit Subfamily GTPase-Mediated Signaling and Neuronal Differentiation and Survival
  • Immunofluorescence Methods in Studies of the GTPase RAN and Its Effectors in Interphase and in Mitotic Cells
  • High Resolution Scanning Electron Microscopy for the Imaging of Nuclear Pore Complexes and Ran-Mediated Transport
  • Effector Recruitment Method to Study Spatially Regulated Activation of Ras and Rho GTPases
  • Real-Time Visualization and Quantification of Native Ras Activation in Single Living Cells
  • Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis
  • Analyzing the Roles of Rho GTPases in Cancer Cell Migration with a Live Cell Imaging 3D-Morphology-Based Assay
  • Analysis of Rho-GTPase-Induced Localization of Nanoscale Adhesions Using Fluorescence Nanoscopy
  • Yeast as a Model for Ras Signaling
  • Methods to Study the Ras2 Protein Activation State and the Subcellular Localization of Ras-GTP in Saccharomyces cerevisiae
  • RAS  Proteins Signaling in the Early Metazoan Dictyostelium discoideum.