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20201012152526.0 |
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190531s2019 xxu| s |||| 0|eng d |
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|a 9781493995325
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| 024 |
7 |
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|a 10.1007/978-1-4939-9532-5
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Protein Lipidation :
|b Methods and Protocols /
|c edited by Maurine E. Linder.
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| 250 |
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|a 1st ed. 2019.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2019.
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| 300 |
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|a XIV, 327 p. 63 illus., 44 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 2009
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| 505 |
0 |
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|a Determination of Protein S-Acylation State by Enhanced Acyl-Switch Methods -- Detection of Heterogeneous Protein S-Acylation in Cells -- Optimization of Metabolic Labeling with Alkyne-Containing Isoprenoid Probes -- Chemical Proteomic Analysis of S-Fatty Acylated Proteins and their Modification Sites -- Direct Analysis of Protein S-Acylation by Mass Spectrometry -- Enrichment of S-Palmitoylated Proteins for Mass Spectrometry Analysis -- Systematic Screening of Depalmitoylating Enzymes and Evaluation of their Activities by the Acyl-PEGyl Exchange Gel-Shift (APEGS) Assay -- Measuring S-Depalmitoylation Activity In Vitro and In Live Cells with Fluorescent Probes -- Dynamic Radiolabeling of S-Palmitoylated Proteins -- Fluorogenic Assays for the Defatty-Acylase Activity of Sirtuins -- Global Profiling of Sirtuin Deacylase Substrates Using Chemical Proteomic Strategy and Validation by Fluorescent Labeling -- siRNA Knockdown of Mammalian zDHHCs and Validation of mRNA Expression by RT-qPCR -- In Vitro Assays to Monitor the Enzymatic Activities of zDHHC Protein Acyltransferases -- Purification of Recombinant zDHHC Proteins Using an Insect Cell Expression System -- Bioinformatic Identification of Functionally and Structurally Relevant Residues and Motifs in Protein S-Acyltransferases -- SwissPalm 2: Protein S-Palmitoylation Database -- Probing Interaction of Lipid-Modified Wnt Protein and Its Receptors by ELISA -- Biochemical Assays for Ghrelin Acylation and Inhibition of Ghrelin O-Acyltransferase -- In Vitro Analysis of Hedgehog Acyltransferase and Porcupine Fatty Acyltransferase Activities -- Production of Farnesylated and Methylated Proteins in an Engineered Insect Cell System -- A Quantitative FRET Assay for the Upstream Cleavage Activity of the Integral Membrane Proteases Human ZMPSTE24 and Yeast Ste24 -- Monitoring RhoGDI Extraction of Lipid-Modified Rho GTPases from Membranes using Click Chemistry -- Purification of the Rhodopsin-Transducin Complex for Structural Studies -- Reconstitution of the Rhodopsin-Transducin Complex into Lipid Nanodiscs.
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| 650 |
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|a Proteins .
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| 650 |
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|a Lipids.
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| 650 |
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|a Mass spectrometry.
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| 650 |
1 |
4 |
|a Protein Science.
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| 650 |
2 |
4 |
|a Lipidology.
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| 650 |
2 |
4 |
|a Mass Spectrometry.
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| 700 |
1 |
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|a Linder, Maurine E.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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