Protein Lipidation : Methods and Protocols /
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edición: | 1st ed. 2019. |
| Colección: | Methods in Molecular Biology,
2009 |
| Materias: |
Tabla de Contenidos:
- Determination of Protein S-Acylation State by Enhanced Acyl-Switch Methods
- Detection of Heterogeneous Protein S-Acylation in Cells
- Optimization of Metabolic Labeling with Alkyne-Containing Isoprenoid Probes
- Chemical Proteomic Analysis of S-Fatty Acylated Proteins and their Modification Sites
- Direct Analysis of Protein S-Acylation by Mass Spectrometry
- Enrichment of S-Palmitoylated Proteins for Mass Spectrometry Analysis
- Systematic Screening of Depalmitoylating Enzymes and Evaluation of their Activities by the Acyl-PEGyl Exchange Gel-Shift (APEGS) Assay
- Measuring S-Depalmitoylation Activity In Vitro and In Live Cells with Fluorescent Probes
- Dynamic Radiolabeling of S-Palmitoylated Proteins
- Fluorogenic Assays for the Defatty-Acylase Activity of Sirtuins
- Global Profiling of Sirtuin Deacylase Substrates Using Chemical Proteomic Strategy and Validation by Fluorescent Labeling
- siRNA Knockdown of Mammalian zDHHCs and Validation of mRNA Expression by RT-qPCR
- In Vitro Assays to Monitor the Enzymatic Activities of zDHHC Protein Acyltransferases
- Purification of Recombinant zDHHC Proteins Using an Insect Cell Expression System
- Bioinformatic Identification of Functionally and Structurally Relevant Residues and Motifs in Protein S-Acyltransferases
- SwissPalm 2: Protein S-Palmitoylation Database
- Probing Interaction of Lipid-Modified Wnt Protein and Its Receptors by ELISA
- Biochemical Assays for Ghrelin Acylation and Inhibition of Ghrelin O-Acyltransferase
- In Vitro Analysis of Hedgehog Acyltransferase and Porcupine Fatty Acyltransferase Activities
- Production of Farnesylated and Methylated Proteins in an Engineered Insect Cell System
- A Quantitative FRET Assay for the Upstream Cleavage Activity of the Integral Membrane Proteases Human ZMPSTE24 and Yeast Ste24
- Monitoring RhoGDI Extraction of Lipid-Modified Rho GTPases from Membranes using Click Chemistry
- Purification of the Rhodopsin-Transducin Complex for Structural Studies
- Reconstitution of the Rhodopsin-Transducin Complex into Lipid Nanodiscs.