Phloem : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Liesche, Johannes. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 2014
Materias:
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245 1 0 |a Phloem :  |b Methods and Protocols /  |c edited by Johannes Liesche. 
250 |a 1st ed. 2019. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2019. 
300 |a XIV, 473 p. 107 illus., 87 illus. in color. :  |b online resource. 
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490 1 |a Methods in Molecular Biology,  |v 2014 
505 0 |a Part 1 : Investigating phloem anatomy -- Optimal preparation of tissue sections for light-microscopic analysis of phloem anatomy -- Transmission electron microscopy of the phloem with minimal artifacts -- Scanning electron microscopy of the phloem -- Non-invasive investigation of phloem structure by 3D synchrotron X-ray microtomography -- Quantification of leaf phloem anatomical features with microscopy -- Methods of phloem visualization - a clear future in sight? -- Part 2 : Visualizing phloem proteins -- Super-resolution microscopy of phloem proteins -- Live-cell imaging of fluorescently-tagged phloem proteins with confocal microscopy -- Immunohistochemical localization of proteins in the phloem: problems and solutions -- Part 3 : Sampling phloem sap -- SStudying phloem loading with EDTA-facilitated phloem exudate collection and analysis -- Measurement of inner bark and leaf osmolality -- Using 13C to quantify phloem transport on tall plants in the field -- Non-invasive determination of phloem transport speed with carbon-14 (14C) -- Detecting rapid changes in carbon transport and partitioning with carbon-11 (11C) -- In vivo veritas: radiotracers in studies of phloem transport of carbohydrate -- Measuring phloem transport velocity in Arabidopsis seedlings using the fluorescent coumarin glucoside, esculin -- Measuring phloem transport velocity on a tissue-level using a phloem-mobile dye -- Part 4 : Investigating mechanism and quantities of phloem loading and unloading -- Quantification of symplasmic phloem loading capacity with live-cell microscopy -- Assessing Long-distance Carbon Partitioning from Photosynthetic Source Leaves to Heterotrophic Sink Organs with Photoassismilated [14C]CO2 -- Measurement of subcellular metabolite concentrations in relation to phloem loading -- Measuring sucrose transporter activity using a protoplast esculin assay -- The AtSUC2 promoter: A powerful tool to study phloem physiology and development -- Part 5 : Phloem physiology -- Using aphids to measure turgor pressure inside sieve elements -- Stem compression: A means to reversibly reduce phloem transport in tree stems -- Methods for assessing the role of phloem transport in plant stress responses -- Part 6 : Theoretical models and microfluidics of phloem function -- Modeling the hydraulic conductivity of phloem sieve elements -- Using a multi-compartmental metabolic model to predict carbon allocation in Arabidopsis thaliana -- A mechanistic model to predict distribution of carbon among multiple sinks -- Using a mathematical model of phloem transport to optimize strategies for crop improvement -- Making microfluidic devices that simulate phloem transport -- Part 7 : Signals in the phloem -- Efficient establishment of interfamily heterograft of Nicotiana benthamiana and Arabidopsis thaliana -- Identification of phloem mobile mRNAs using the Solanacea heterograft system -- Analyzing and predicting phloem mobility of macromolecules with an online database -- Measurement of electropotential waves in intact phloem sieve elements using microelectrodes -- Measurement of electropotential waves in intact sieve elements using aphids as bioelectrodes -- What can cell culture systems reveal about sieve element differentiation? 
650 0 |a Plant science. 
650 0 |a Botany. 
650 1 4 |a Plant Sciences. 
700 1 |a Liesche, Johannes.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
900 |a Libro descargado a ALEPH en bloque (proveniente de proveedor)