Phloem : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Liesche, Johannes. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 2014
Materias:
Tabla de Contenidos:
  • Part 1 : Investigating phloem anatomy
  • Optimal preparation of tissue sections for light-microscopic analysis of phloem anatomy
  • Transmission electron microscopy of the phloem with minimal artifacts
  • Scanning electron microscopy of the phloem
  • Non-invasive investigation of phloem structure by 3D synchrotron X-ray microtomography
  • Quantification of leaf phloem anatomical features with microscopy
  • Methods of phloem visualization - a clear future in sight?
  • Part 2 : Visualizing phloem proteins
  • Super-resolution microscopy of phloem proteins
  • Live-cell imaging of fluorescently-tagged phloem proteins with confocal microscopy
  • Immunohistochemical localization of proteins in the phloem: problems and solutions
  • Part 3 : Sampling phloem sap
  • SStudying phloem loading with EDTA-facilitated phloem exudate collection and analysis
  • Measurement of inner bark and leaf osmolality
  • Using 13C to quantify phloem transport on tall plants in the field
  • Non-invasive determination of phloem transport speed with carbon-14 (14C)
  • Detecting rapid changes in carbon transport and partitioning with carbon-11 (11C)
  • In vivo veritas: radiotracers in studies of phloem transport of carbohydrate
  • Measuring phloem transport velocity in Arabidopsis seedlings using the fluorescent coumarin glucoside, esculin
  • Measuring phloem transport velocity on a tissue-level using a phloem-mobile dye
  • Part 4 : Investigating mechanism and quantities of phloem loading and unloading
  • Quantification of symplasmic phloem loading capacity with live-cell microscopy
  • Assessing Long-distance Carbon Partitioning from Photosynthetic Source Leaves to Heterotrophic Sink Organs with Photoassismilated [14C]CO2
  • Measurement of subcellular metabolite concentrations in relation to phloem loading
  • Measuring sucrose transporter activity using a protoplast esculin assay
  • The AtSUC2 promoter: A powerful tool to study phloem physiology and development
  • Part 5 : Phloem physiology
  • Using aphids to measure turgor pressure inside sieve elements
  • Stem compression: A means to reversibly reduce phloem transport in tree stems
  • Methods for assessing the role of phloem transport in plant stress responses
  • Part 6 : Theoretical models and microfluidics of phloem function
  • Modeling the hydraulic conductivity of phloem sieve elements
  • Using a multi-compartmental metabolic model to predict carbon allocation in Arabidopsis thaliana
  • A mechanistic model to predict distribution of carbon among multiple sinks
  • Using a mathematical model of phloem transport to optimize strategies for crop improvement
  • Making microfluidic devices that simulate phloem transport
  • Part 7 : Signals in the phloem
  • Efficient establishment of interfamily heterograft of Nicotiana benthamiana and Arabidopsis thaliana
  • Identification of phloem mobile mRNAs using the Solanacea heterograft system
  • Analyzing and predicting phloem mobility of macromolecules with an online database
  • Measurement of electropotential waves in intact phloem sieve elements using microelectrodes
  • Measurement of electropotential waves in intact sieve elements using aphids as bioelectrodes
  • What can cell culture systems reveal about sieve element differentiation?