Phloem : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edition: | 1st ed. 2019. |
| Series: | Methods in Molecular Biology,
2014 |
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Table of Contents:
- Part 1 : Investigating phloem anatomy
- Optimal preparation of tissue sections for light-microscopic analysis of phloem anatomy
- Transmission electron microscopy of the phloem with minimal artifacts
- Scanning electron microscopy of the phloem
- Non-invasive investigation of phloem structure by 3D synchrotron X-ray microtomography
- Quantification of leaf phloem anatomical features with microscopy
- Methods of phloem visualization - a clear future in sight?
- Part 2 : Visualizing phloem proteins
- Super-resolution microscopy of phloem proteins
- Live-cell imaging of fluorescently-tagged phloem proteins with confocal microscopy
- Immunohistochemical localization of proteins in the phloem: problems and solutions
- Part 3 : Sampling phloem sap
- SStudying phloem loading with EDTA-facilitated phloem exudate collection and analysis
- Measurement of inner bark and leaf osmolality
- Using 13C to quantify phloem transport on tall plants in the field
- Non-invasive determination of phloem transport speed with carbon-14 (14C)
- Detecting rapid changes in carbon transport and partitioning with carbon-11 (11C)
- In vivo veritas: radiotracers in studies of phloem transport of carbohydrate
- Measuring phloem transport velocity in Arabidopsis seedlings using the fluorescent coumarin glucoside, esculin
- Measuring phloem transport velocity on a tissue-level using a phloem-mobile dye
- Part 4 : Investigating mechanism and quantities of phloem loading and unloading
- Quantification of symplasmic phloem loading capacity with live-cell microscopy
- Assessing Long-distance Carbon Partitioning from Photosynthetic Source Leaves to Heterotrophic Sink Organs with Photoassismilated [14C]CO2
- Measurement of subcellular metabolite concentrations in relation to phloem loading
- Measuring sucrose transporter activity using a protoplast esculin assay
- The AtSUC2 promoter: A powerful tool to study phloem physiology and development
- Part 5 : Phloem physiology
- Using aphids to measure turgor pressure inside sieve elements
- Stem compression: A means to reversibly reduce phloem transport in tree stems
- Methods for assessing the role of phloem transport in plant stress responses
- Part 6 : Theoretical models and microfluidics of phloem function
- Modeling the hydraulic conductivity of phloem sieve elements
- Using a multi-compartmental metabolic model to predict carbon allocation in Arabidopsis thaliana
- A mechanistic model to predict distribution of carbon among multiple sinks
- Using a mathematical model of phloem transport to optimize strategies for crop improvement
- Making microfluidic devices that simulate phloem transport
- Part 7 : Signals in the phloem
- Efficient establishment of interfamily heterograft of Nicotiana benthamiana and Arabidopsis thaliana
- Identification of phloem mobile mRNAs using the Solanacea heterograft system
- Analyzing and predicting phloem mobility of macromolecules with an online database
- Measurement of electropotential waves in intact phloem sieve elements using microelectrodes
- Measurement of electropotential waves in intact sieve elements using aphids as bioelectrodes
- What can cell culture systems reveal about sieve element differentiation?