RNAi and microRNA-Mediated Gene Regulation in Stem Cells : Methods, Protocols, and Applications /

RNAi and microRNA-Mediated Gene Regulation in Stem Cells : Methods, Protocols, and Applications /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Zhang, Baohong. (Editor ), Stellwag, Edmund J. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2010.
Edición:1st ed. 2010.
Colección:Methods in Molecular Biology, 650
Materias:
Cell biology.
Biochemistry.
Human genetics.
Stem cells.
Gene therapy.
Cell Biology.
Biochemistry, general.
Human Genetics.
Stem Cells.
Gene Therapy.
Tabla de Contenidos:
  • RNAi in Stem Cells: Current Status and Future Perspectives
  • A Brief Introduction to RNAi and MicroRNAs in Stem Cells
  • RNA Interference
  • Forward RNAi Screens in Human Stem Cells
  • A Recessive Genetic Screen for Components of the RNA Interference Pathway in Mouse Embryonic Stem Cells
  • Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells
  • Establishing Efficient siRNA Knockdown in Stem Cells Using Fluorescent Oligonucleotides
  • Loss-of-Function Studies in Mouse Embryonic Stem Cells Using the pHYPER shRNA Plasmid Vector
  • Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8
  • Silencing of Rho-GDI? by RNAi Promotes the Differentiation of Neural Stem Cells
  • RNAi Knockdown of Redox Signaling Protein Ape1 in the Differentiation of Mouse Embryonic Stem Cells
  • Proteins Involved in Cell Migration from Glioblastoma Neurospheres Analyzed by Overexpression and siRNA-Mediated Knock-Down
  • An Efficient Transfection Method for Mouse Embryonic Stem Cells
  • Semi-quantitative Analysis of Transient Single-Cell Gene Expression in Embryonic Stem Cells by Femtoinjection
  • MicroRNAs
  • Preparation and Analysis of MicroRNA Libraries Using the Illumina Massively Parallel Sequencing Technology
  • Assessing In Vivo MicroRNA Function in the Germline Stem Cells of the Drosophila Ovary
  • Monitoring MicroRNA Expression During Embryonic Stem-Cell Differentiation Using Quantitative Real-Time PCR (qRT-PCR)
  • Engineering Human Mesenchymal Stem Cells to Release Adenosine Using miRNA Technology
  • Efficient Gene Knockdowns in Mouse Embryonic Stem Cells Using MicroRNA-Based shRNAs.

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