Plant-Pathogen Interactions : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Birch, Paul. (Editor), Jones, John T. (Editor), Bos, Jorunn I.B. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edition:2nd ed. 2014.
Series:Methods in Molecular Biology, 1127
Subjects:
Table of Contents:
  • Galaxy as a Platform for Identifying Candidate Pathogen Effectors
  • Bioinformatic analysis of expression data to identify effector candidates
  • Two-dimensional data binning for the analysis of genome architecture in filamentous plant pathogens and other eukaryotes
  • On The Statistics Of Identifying Candidate Pathogen Effectors
  • High-throughput imaging of plant immune responses
  • In vivo protein-protein interaction studies with BiFC: conditions, cautions and caveats
  • Particle bombardment-mediated transient expression to identify localization signals in plant disease resistance proteins and target sites for the proteolytic activity of pathogen effectors
  • Purification of fungal haustoria from infected plant tissue by flow cytometry
  • Functional Characterisation of Nematode Effectors in Plants
  • Silencing of aphid genes by feeding on stable transgenic Arabidopsis thaliana
  • Leaf-disc assay based on transient over-expression in Nicotiana benthamiana to allow functional screening of candidate effectors from aphids
  • A growth quantification assay for Hyaloperonospora arabidopsidis isolates in Arabidopsis thaliana
  • Simple quantification of in planta fungal biomass
  • Virus-induced gene silencing and Agrobacterium tumefaciens-mediated transient expression in Nicotiana tabacum
  • DIGE-ABPP by click chemistry: Pairwise comparison of serine hydrolase activities from the apoplast of infected plants
  • A Simple and Fast Protocol for the Protein Complex Immunoprecipitation (Co-IP) of Effector - Host Protein Complexes
  • An Arabidopsis and Tomato Mesophyll Protoplast System for fast Identification of early MAMP-triggered Immunity-Suppressing Effectors
  • Production of RXLR effector proteins for structural analysis by X-ray crystallography
  • The Do's and Don'ts of effectoromics
  • Protoplast cell death assay to study Magnaporthe oryzae AVR gene function in rice
  • A Bacterial Type III Secretion Based Delivery System for Functional Assays of Fungal Effectors in Cereals
  • Capture arrays to annotate resistance genes in plant genomes and to accelerate plant resistance gene discovery.