Natural Killer Cell Protocols : Cellular and Molecular Methods /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Campbell, Kerry S. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2010.
Edición:2nd ed. 2010.
Colección:Methods in Molecular Biology, 612
Materias:
Tabla de Contenidos:
  • Purification of Human NK Cell Developmental Intermediates from Lymph Nodes and Tonsils
  • In Vitro Development of Human Killer–Immunoglobulin Receptor-Positive NK Cells
  • Subset Analysis of Human and Mouse Mature NK Cells
  • Assessing Licensing of NK Cells
  • Use of Stem Cell Radiation Chimeras to Analyze How Domains of Specific Proteins Impact on Murine NK Cell Development In Vivo
  • Use of Transfected Drosophila S2 Cells to Study NK Cell Activation
  • Natural Killer Cell Conjugate Assay Using Two-Color Flow Cytometry
  • Studying NK Cell/Dendritic Cell Interactions
  • Analysis of the NK Cell Immunological Synapse
  • Measuring Intracellular Calcium Signaling in Murine NK Cells by Flow Cytometry
  • Intracellular Staining for Analysis of the Expression and Phosphorylation of Signal Transducers and Activators of Transcription (STATs) in NK Cells
  • A Model System for Studying NK Cell Receptor Signaling
  • Expression of cDNAs in Human Natural Killer Cell Lines by Retroviral Transduction
  • Lentiviral Gene Transduction in Human and Mouse NK Cell Lines
  • of shRNAs into Human NK-Like Cell Lines with Retrovirus
  • of shRNAs into Primary NK Cells with Lentivirus
  • Methods to Identify and Characterize Different NK Cell Receptors and Their Ligands
  • Generating NK Cell Receptor-Fc Chimera Proteins from 293T Cells and Considerations of Appropriate Glycosylation
  • Identification of NK Cell Receptor Ligands Using a Signaling Reporter System
  • Determining Ligand Specificity of Ly49 Receptors
  • Probing the Interactions of NK Cell Receptors with Ligand Expressed in trans and cis
  • A Simple Method to Measure NK Cell Cytotoxicity In Vivo
  • Functional Analysis of Human NK Cells by Flow Cytometry
  • Analysis of the KIR Repertoire in Human NK Cells by Flow Cytometry
  • KIR Genotyping by Multiplex PCR-SSP
  • Identification and Analysis of Novel Transcripts and Promoters in the Human Killer Cell Immunoglobulin-like Receptor (KIR?) Genes
  • Use of Inbred Mouse Strains to Map Recognition Receptors of MCMV Infected Cells in the NK Cell Gene Locus
  • Studying NK Cell Responses to Ectromelia Virus Infections in Mice
  • Activation of Human NK Cells by Malaria-Infected Red Blood Cells
  • Natural Killer Cells in Human Pregnancy
  • Analysis of Uterine Natural Killer Cells in Mice
  • Isolation of NK Cells and NK-Like Cells from the Intestinal Lamina Propria.