Poly(ADP-Ribose) Polymerase : Methods and Protocols /
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2017.
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| Edición: | 2nd ed. 2017. |
| Colección: | Methods in Molecular Biology,
1608 |
| Materias: |
Tabla de Contenidos:
- Quantitation of Poly(ADP-ribose) by Isotope Dilution Mass Spectrometry
- Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemistry in Human Biopsies
- Detecting and Quantifying pADPr In Vivo
- Compartment-Specific Poly-ADP-Ribose Formation as a Biosensor for Subcellular NAD Pools
- Cell Cycle Resolved Measurements of Poly(ADP-ribose) Formation and DNA Damage Signaling by Quantitative Image-Based Cytometry
- Detecting Protein ADP-Ribosylation Using a Clickable Aminooxy Probe
- ADP-Ribosylated Peptide Enrichment and Site Identification: The Phosphodiesterase-Based Method
- Using Clickable NAD+ Analogues to Label Substrate Proteins of PARPs
- Identification of Protein Substrates of Specific PARP Enzymes Using Analog-Sensitive PARP Mutants and a ‘Clickable’ NAD+ Analog
- Identification of ADP-Ribose Acceptor Sites on In Vitro Modified Proteins by Liquid Chromatography – Tandem Mass Spectrometry
- Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography –Tandem Mass Spectrometry
- Poly(ADP-Ribose)-Dependent Chromatin Remodeling in DNA Repair
- Methods to Assess the Role of Poly(ADP-Ribose) Polymerases in Regulating Mitochondrial Oxidative Function
- Approaches for Investigating Translational Regulation Controlled by PARP1: Biotin-Based UV-Crosslinking and Luciferase Reporter Assay
- Methodology to Identify Poly-ADP-Ribose Polymerase 1 (PARP1) – mRNA Targets by PAR-CLiP
- Biochemical and Biophysical Methods for Analysis of Poly(ADP-Ribose) Polymerase 1 and Its Interactions with Chromatin
- PARP-1 Interaction with and Activation by Histones and Nucleosomes
- Strategies Employed for the Development of PARP Inhibitors
- High-Throughput Colorimetric Assay for Identifying PARP-1 Inhibitors Using a Large Small-Molecule Collection
- Testing PARP Inhibitors Using a Subcutaneous Murine Xenograft Model
- In Vitro Long Term Proliferation Assays to Study Antiproliferative Effects of PARP Inhibitors on Cancer Cells
- Use of Inosine Monophosphate Dehydrogenase Activity Assay to Determine the Specificity of PARP-1 Inhibitors
- The Use of PARP Inhibitors in Cancer Therapy: Use as Adjuvant with Chemotherapy or Radiotherapy, Use as a Single Agent in Susceptible Patients, and Techniques Used to Identify Susceptible Patients
- Purification of Recombinant Human PARP-3
- Purification of Recombinant Human PARG and Activity Assays
- Studying Catabolism of Protein ADP-Ribosylation
- Purification of DNA Damage-Dependent PARPs from E. coli for Structural and Biochemical Analysis
- Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach
- Computational and Experimental Studies of ADP-Ribosylation.