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100301s1998 xxu| s |||| 0|eng d |
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|a 9781592595709
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| 024 |
7 |
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|a 10.1385/0896034941
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a RNA Isolation and Characterization Protocols /
|c edited by Ralph Rapley, David L. Manning.
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| 250 |
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|a 1st ed. 1998.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1998.
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| 300 |
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|a XIII, 264 p. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 86
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| 505 |
0 |
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|a to Isolating RNA -- Large and Small Scale RNA Preparations from Eukaryotic Cells -- An Improved Rapid Method of Isolating RNA from Cultured Cells -- Isolating RNA with the Cationic Surfactant, Catrimox-14 -- RNA Extraction from Formalin-Fixed and Paraffin-Embedded Tissues -- Extraction and Purification of RNA from Plant Tissue Enriched in Polysaccharides -- Isolation of Plant Mitochondrial RNA from Green Leaves -- Extraction of RNA from Fresh and Frozen Blood -- Isolation of Total RNA from Bacteria -- Isolation of Total RNA from Tissues or Cell Lines -- Isolation of Messenger RNA -- UV Spectrophotometric Analysis of Ribonucleic Acids -- Formaldehyde Gel Electrophoresis of Total RNA -- Preparation of RNA Dot-Blots -- Nonradioactive Northern Blotting -- The Use of RNA Probes for the Analysis of Gene Expression -- Analysis of RNA by Northern Blotting Using Riboprobes -- RNA Quantitative Analysis from Fixed and Paraffin-Embedded Tissues -- Quantitative Analysis of RNA Species by PCR and Solid-Phase Minisequencing -- Preparation of Tissue Sections and Slides for mRNA Hybridization -- Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes -- One-Tube RT-PCR with Sequence-Specific Primers -- Identification of Differentially Expressed Genes by Nonradioactive Differential Display of Messenger RNA -- Characterization of RNA Using Continuous RT-PCR Coupled with ELOSA -- Gene Expression Analysis by CD-RT-PCR Eric de Kant -- Primer Extension Analysis of mRNA -- S1 Mapping Using Single-Stranded DNA Probes -- Measurements of Rate of Transcription in Isolated Nuclie by Nuclear “Run-Off” Assay -- Transcription In Vitro Using Bacteriophage RNA Polymerases -- In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System -- In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System -- The Xenopus Egg Extract Translation System -- Purification and Characterization of Viral dsRNA Genome Profiles by Crosshybridization.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Rapley, Ralph.
|e editor.
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| 700 |
1 |
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|a Manning, David L.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|