RNA Isolation and Characterization Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1998.
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| Edición: | 1st ed. 1998. |
| Colección: | Methods in Molecular Biology,
86 |
| Materias: |
Tabla de Contenidos:
- to Isolating RNA
- Large and Small Scale RNA Preparations from Eukaryotic Cells
- An Improved Rapid Method of Isolating RNA from Cultured Cells
- Isolating RNA with the Cationic Surfactant, Catrimox-14
- RNA Extraction from Formalin-Fixed and Paraffin-Embedded Tissues
- Extraction and Purification of RNA from Plant Tissue Enriched in Polysaccharides
- Isolation of Plant Mitochondrial RNA from Green Leaves
- Extraction of RNA from Fresh and Frozen Blood
- Isolation of Total RNA from Bacteria
- Isolation of Total RNA from Tissues or Cell Lines
- Isolation of Messenger RNA
- UV Spectrophotometric Analysis of Ribonucleic Acids
- Formaldehyde Gel Electrophoresis of Total RNA
- Preparation of RNA Dot-Blots
- Nonradioactive Northern Blotting
- The Use of RNA Probes for the Analysis of Gene Expression
- Analysis of RNA by Northern Blotting Using Riboprobes
- RNA Quantitative Analysis from Fixed and Paraffin-Embedded Tissues
- Quantitative Analysis of RNA Species by PCR and Solid-Phase Minisequencing
- Preparation of Tissue Sections and Slides for mRNA Hybridization
- Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes
- One-Tube RT-PCR with Sequence-Specific Primers
- Identification of Differentially Expressed Genes by Nonradioactive Differential Display of Messenger RNA
- Characterization of RNA Using Continuous RT-PCR Coupled with ELOSA
- Gene Expression Analysis by CD-RT-PCR Eric de Kant
- Primer Extension Analysis of mRNA
- S1 Mapping Using Single-Stranded DNA Probes
- Measurements of Rate of Transcription in Isolated Nuclie by Nuclear “Run-Off” Assay
- Transcription In Vitro Using Bacteriophage RNA Polymerases
- In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System
- In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System
- The Xenopus Egg Extract Translation System
- Purification and Characterization of Viral dsRNA Genome Profiles by Crosshybridization.