Plant Kinases : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Dissmeyer, Nico. (Editor ), Schnittger, Arp. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edición:1st ed. 2011.
Colección:Methods in Molecular Biology, 779
Materias:
Tabla de Contenidos:
  • Guide to the Book Plant Kinases
  • The Age of Protein Kinases
  • Expression and Purification of Active Protein Kinases from Wheat Germ Extracts
  • Measurement of Plant Cyclin-Dependent Kinase Activity Using Immunoprecipitation-Coupled and Affinity Purification-Based Kinase Assays and the Baculovirus Expression System
  • Mitogen-Activated Protein Kinase Activity and Reporter Gene Assays in Plants
  • Use of Phospho-site Substitutions to Analyze the Biological Relevance of Phosphorylation Events in Regulatory Networks
  • Bacterial Assay to Study Plant Sensor Histidine Kinases.-  Substrate Analysis of Arabidopsis PP2C-type Protein Phosphatase
  • Modulating and Monitoring MAPK Activity During Programmed Cell Death in Pollen
  • Sensitizing Plant Protein Kinases to Specific Inhibition by ATP-Competitive Molecules
  • Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization
  • Quantifying Degradation Rates of Trans-Membrane Receptor Kinases
  • Fluorescence Correlation Spectroscopy and Fluorescence Recovery after Photobleaching to Study Receptor Kinase Mobility in planta
  • Bimolecular-Fluorescence Complementation Assay to Monitor Kinase-Substrate Interactions in vivo
  • Chemical Genetic Analysis of Protein Kinase Function in Plants
  • Modified Metal Oxide Affinity Enrichment Combined with 2D-PAGE and Analysis of Phosphoproteomes
  • Phosphoproteomics Using iTRAQ.