RNA : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2011.
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| Edición: | 1st ed. 2011. |
| Colección: | Methods in Molecular Biology,
703 |
| Materias: |
Tabla de Contenidos:
- The Transcriptional Landscape
- Working with RNA
- Synthesis of RNA by In vitro Transcription
- Efficient Poly(A)+ RNA Selection Using LNA Oligo(T) Capture
- Genome Browsers
- Web-Based Tools for Studying RNA Structure and Function
- Northern Blotting Analysis
- Rapid Amplification of cDNA Ends (RACE)
- Fractionation of mRNA Based on the Length of the Poly(A) Tail
- Analysis of Mutations that Influence Pre-mRNA Splicing
- S1 Nuclease Analysis of Alternatively Spliced mRNA
- Promises and Challenges in Developing RNAi as a Research Tool and Therapy
- Inhibition of Gene Function in Mammalian Cells Using Short-Hairpin RNA (shRNA)
- Validation of RNAi by Real Time PCR
- Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method
- The Post-Transcriptional Operon
- RIP-Chip Analysis: RNA-Binding Protein Immunoprecipitation-Microarray (Chip) Profiling
- Isolation of RNP Granules
- Electrophoretic Mobility Shift Assay for Characterizing RNA-Protein Interaction
- Polysome Analysis and RNA Purification from Sucrose Gradients
- Prediction of Targets for microRNAs
- Outsourcing of Experimental Work
- ESM1: In-Line Probing Analysis of Riboswitches
- ESM2: Analysis of the RNA Backbone: Structural Analysis of Riboswitches by In-Line Probing and Selective 2'-Hydroxyl Acylation and Primer Extension (SHAPE).