Protein Electrophoresis : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2012.
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| Edición: | 1st ed. 2012. |
| Colección: | Methods in Molecular Biology,
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| Materias: |
Tabla de Contenidos:
- How It All Began
- A Personal History of Gel-Electrophoresis
- Introduction to Protein Electrophoresis
- Protein Quantification Methods to Determine Protein Concentration Prior to Electrophoresis
- Protein Solubilization: Attend to the Choice of Lysis Buffer
- Native Polyacrylamide Gels
- Cationic Electrophoresis
- SARCOSYL-PAGE: A New Electrophoretic Method for the Separation and Immunological Detection of PEGylated Proteins
- Tricine-SDS-PAGE
- Analysis of Protein Glycation using Phenylboronate Acrylamide Gel Electrophoresis
- Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins
- Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis
- A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples
- Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies
- Microchip Electrophoresis, with respect to “Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheimer’s Disease”
- A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols
- Peptide Fractionation by SDS-Free Polyacrylamide Gel Electrophoresis for Proteomic Analysis via DF-PAGE
- Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass
- SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting
- Two Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing
- Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids
- Native Flat-Bed Isoelectric Focusing for Determining Antibody Clonotype Distribution
- Two-Dimensional Gel Electrophoresis: Glass Tube-Based IEF Followed by SDS-PAGE
- One-Dimensional and Two-Dimensional Immobilized Metal Affinity Electrophoresis
- Two-Dimensional Difference Gel Electrophoresis
- Protein Identification on Archived 2-D Gels
- Diagonal Electrophoresis for the Detection of Protein Disulfides
- Two-Dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes
- Immunoelectrophoresis: A Method with Many Faces
- Cell Surface Protein Biotinylation for SDS PAGE Analysis
- A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization
- Gel Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane Proteome
- Interactions of Hemoglobin in Live Red Blood Cells Measured by the Electrophoresis Release Test
- Extraction of Proteins from Gels - A Brief Review
- A New Algorithm for the Simulation of SDS 2D-PAGE Datasets
- Isolation of Proteins from Polyacrylamide Gels
- Gel Drying Methods
- Long-Term, Buffer-Less Wet Gel Storage in Non-Sealed Polyethylene Bags
- A Brief Review of Other Notable Electrophoretic Methods
- Protein Stains and Applications
- Coomassie-Brilliant Blue Staining of Polyacrylamide Gels
- Accelerated Coomassie Blue Protein Staining and Destaining with Application of Heat
- Silver Staining Techniques of Polyacrylamide Gels
- Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis
- Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry
- TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots after PAGE
- Microwave Assisted Protein Staining, De-Staining and In-Gel / In-Solution Digestion of Proteins
- Electrophoretic Separation and Detection of Metalloproteins by X-ray Fluorescence Mapping
- Fluorescent Staining of Gels
- A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta During Western Blot Analysis
- Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain
- Spicy SDS-PAGE Gels: Curcumin/Turmeric as an Environment-Friendly Protein Stain
- In-Gel Staining of Proteins in Native Poly Acryl Amide Gel Electrophoresis Using meso-Tetrakis(4-sulfonato phenyl)porphyrin
- Rapid Detection of Proteins in Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black
- Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gels
- Coomassie Brilliant Blue Removal/Disposal from Gel Destain and Used Gel Stain in an Environment-Friendly Manner
- A Brief Review of Other Notable Protein Detection Methods on Acrylamide Gels
- Ultra-Sensitive Protein Detection and Imaging: Comparison of Lumitein™, ProteoSilver™, SYPRO® Ruby, and Coomassie® Brilliant Blue Gel Stains
- Common Artifacts and Mistakes Made in Electrophoresis.