Peroxisomes : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Schrader, Michael. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1595
Materias:
Tabla de Contenidos:
  • Isolation of Peroxisomes from Rat Liver and Cultured Hepatoma Cells by Density Gradient Centrifugation
  • Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes
  • Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays
  • Isolation of Native Soluble and Membrane-Bound Protein Complexes from Yeast Saccharomyces Cerevisiae
  • Method for Measurement of Peroxisomal Very Long-Chain Fatty Acid Beta-Oxidation and de novo C26:0 Synthesis Activity in Living Cells Using Stable-Isotope Labelled Docosanoic Acid
  • Analysis of Plasmalogen Synthesis in Cultured Cells
  • Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies
  • siRNA-Mediated Silencing of Peroxisomal Genes in Mammalian Cells
  • Dual Reporter Systems for the Analysis of Translational Readthrough in Mammals
  • Cytochemical Detection of Peroxisomes in Light and Electron Microscopy with 3,3’-diaminobenzidine
  • Cryo-Immuno Electron Microscopy of Peroxisomal Marker Proteins
  • Detection and Immunolabelling of Peroxisomal Proteins
  • Labelling of Peroxisomes for Live Cell Imaging in the Filamentous Fungus Ustilago Maydis
  • Quantitative Monitoring of Subcellular Redox Dynamics in Living Mammalian Cells Using RoGFP2-based Probes
  • KillerRed as a Tool to Study the Cellular Responses to Peroxisome-Derived Oxidative Stress
  • Determination of Peroxisomal pH in Living Mammalian Cells Using pHRed
  • In Cellulo Approaches to Study Peroxisomal Protein Import – Yeast Immunofluorescence Microscopy
  • Blue Native Page: Applications to Study Peroxisome Biogenesis
  • In Vitro PMP Import Analysis Using Cell-Free Synthesized PMP and Isolated Peroxisomes
  • Peroxisomal Membrane and Matrix Protein Import Using a Semi-Intact Mammalian Cell System
  • The Use of Glycosylation Tags as Reporters for Protein Entry into the Endoplasmic Reticulum in Yeast and Mammalian Cells
  • Detection of Ubiquitinated Peroxisomal Proteins in Yeast
  • Assessing Pexophagy in Mammalian Cells
  • Experimental Systems to Study Yeast Pexophagy
  • Flow Cytometric Analysis of the Expression Pattern of Peroxisomal Proteins, Abcd1, Abcd2, and Abcd3 in BV-2 Murine Microglial Cells
  • Study of Peroxisomal Protein Phosphorylation by Functional Proteomics
  • Analysis of Peroxisomal β-Oxidation during Storage Oil Mobilization in Arabidopsis Thaliana Seedlings
  • Peroxisome Mini-Libraries - Systematic Approaches to Study Peroxisomes Made Easy
  • Generation of Peroxisome-Deficient Somatic Animal Cell Mutants
  • Clinical and Laboratory Diagnosis of Peroxisomal Disorders.