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02672nam a22003255i 4500 |
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20210909113103.0 |
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cr nn 008mamaa |
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141006s2015 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493915941
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| 024 |
7 |
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|a 10.1007/978-1-4939-1594-1
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Nuclear Reprogramming :
|b Methods and Protocols /
|c edited by Nathalie Beaujean, Hélène Jammes, Alice Jouneau.
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| 250 |
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|a 2nd ed. 2015.
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| 260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2015.
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| 300 |
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|a XI, 285 p. 68 illus., 43 illus. in color. :
|b online resource.
|
| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1222
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| 505 |
0 |
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|a Nuclear Transfer in the Mouse -- Nuclear Transfer in the Rabbit -- Nuclear Transfer in Ruminants -- Nuclear Transfer and Transgenesis in the Pig -- Embryonic Stem Cell-Somatic Cell Fusion and Post-Fusion Enucleation -- Analysis of Nuclear Reprogramming Following Nuclear Transfer to Xenopus Oocyte -- Assessing the Quality of Donor Cells: Karyotyping Methods -- Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer -- Fluorescent Immunodetection of Epigenetic Modifications on Pre-Implantation Mouse Embryos -- Visualization of Epigenetic Modifications in Preimplantation Embryos -- Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer -- Analysis of Nucleolar Morphology and Protein Localization as an Indicator of Nuclear Reprogramming -- Assessment of Cell Lineages and Cell Death in Blastocysts by Immunostaining -- Gene Expression Analysis in Early Embryos through Reverse Transcription Quantitative PCR (RT-qPCR) -- Studying Bovine Early Embryo Transcriptome by Microarray -- Methylation of Specific Regions: Bisulfite-Sequencing at the Single Oocyte or 2-Cell Embryo Level -- Micro Chromatin Immunoprecipitation (ChIP) from Early Mammalian Embryos -- Assessing Reprogramming by Chimera Formation and Tetraploid Complementation -- Whole-Mount In Situ Hybridization to Assess Advancement of Development and Embryo Morphology -- Genome-Wide Analysis of Methylation in Bovine Clones by Methylated DNA Immunoprecipitation (MeDIP).
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| 650 |
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0 |
|a Cell biology.
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| 650 |
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0 |
|a Genetic engineering.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Genetic Engineering.
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| 700 |
1 |
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|a Beaujean, Nathalie.
|e editor.
|
| 700 |
1 |
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|a Jammes, Hélène.
|e editor.
|
| 700 |
1 |
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|a Jouneau, Alice.
|e editor.
|
| 710 |
2 |
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|a SpringerLink (Online service)
|
| 773 |
0 |
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|t Springer eBooks
|