Nuclear Reprogramming : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Edición: | 2nd ed. 2015. |
| Colección: | Methods in Molecular Biology,
1222 |
| Materias: |
Tabla de Contenidos:
- Nuclear Transfer in the Mouse
- Nuclear Transfer in the Rabbit
- Nuclear Transfer in Ruminants
- Nuclear Transfer and Transgenesis in the Pig
- Embryonic Stem Cell-Somatic Cell Fusion and Post-Fusion Enucleation
- Analysis of Nuclear Reprogramming Following Nuclear Transfer to Xenopus Oocyte
- Assessing the Quality of Donor Cells: Karyotyping Methods
- Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer
- Fluorescent Immunodetection of Epigenetic Modifications on Pre-Implantation Mouse Embryos
- Visualization of Epigenetic Modifications in Preimplantation Embryos
- Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer
- Analysis of Nucleolar Morphology and Protein Localization as an Indicator of Nuclear Reprogramming
- Assessment of Cell Lineages and Cell Death in Blastocysts by Immunostaining
- Gene Expression Analysis in Early Embryos through Reverse Transcription Quantitative PCR (RT-qPCR)
- Studying Bovine Early Embryo Transcriptome by Microarray
- Methylation of Specific Regions: Bisulfite-Sequencing at the Single Oocyte or 2-Cell Embryo Level
- Micro Chromatin Immunoprecipitation (ChIP) from Early Mammalian Embryos
- Assessing Reprogramming by Chimera Formation and Tetraploid Complementation
- Whole-Mount In Situ Hybridization to Assess Advancement of Development and Embryo Morphology
- Genome-Wide Analysis of Methylation in Bovine Clones by Methylated DNA Immunoprecipitation (MeDIP).