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130517s2013 xxu| s |||| 0|eng d |
| 020 |
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|a 9781627034449
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| 024 |
7 |
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|a 10.1007/978-1-62703-444-9
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Neural Development :
|b Methods and Protocols /
|c edited by Renping Zhou, Lin Mei.
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| 250 |
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|a 1st ed. 2013.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2013.
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| 300 |
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|a XIII, 347 p. 59 illus., 48 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1018
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| 505 |
0 |
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|a Isolation and Maintenance of Cortical Neural Progenitor Cells In Vitro -- Neural Induction with a Dopaminergic Phenotype from Human Pluripotent Stem Cells Through a Feeder-Free Floating Aggregation Culture -- Nucleoside Analog Labeling of Neural Stem Cells and Their Progeny -- Culture of Dissociated Hippocampal Neurons -- Culturing Mouse Cerebellar Granule Neurons -- Preparation of Primary Cultured Dopaminergic Neurons from Mouse Brain -- Culturing Astoryctes from Postnatal Rats -- Culturing Oligodendrocyte Lineage Cells from Neonatal Rats -- Isolation and Culture of Schwann Cells -- DNA Transfection: Calcium Phosphate Method -- The Gene-Gun Approach for Transfection and Labeling of Cells in Brain Slices -- Lentiviral Vector Production, Titration, and Transduction of Primary Neurons -- In-Ovo Electroporation in Embryonic Chick Spinal Cords -- Gene Transfer in Developing Chick Embryos: In Ovo Electroporation -- In Utero Electroporation in Mice -- General Introduction to In Situ Hybridization Protocol Using Non-Radioactively Labeled Probes to Detect mRNAs on Tissue Sections -- Immunofluorescence Staining with Frozen Mouse or Chick Embryonic Tissue Sections -- β-Galactosidase Staining of LacZ Fusion Proteins in Whole Tissue Preparations -- Chromatin Immunoprecipitation Assay of Brain Tissues Using Percoll Gradient-Purified Nuclei -- In Vivo Dual Luciferase Reporter Assay with Chick Neural Tube In Ovo Electroporation System -- Growth Cone Collapse Assay -- The Stripe Assay: Studying Growth Preference and Axon Guidance on Binary Choice Substrates In Vitro -- Microcontact Printing of Substrate-Bound Protein Patterns for Cell- and Tissue Culture -- Semi-Automated Analysis of Dendrite Morphology in Cell Culture -- Monitoring Synaptic Plasticity by Imaging AMPA Receptor Content and Dynamics on Dendritic Spines -- Morphological Analysis of Neuromuscular Junctions by Immunofluorescent Staining of Whole-Mount Mouse Diaphragms- Routine Histology Techniques for the Developing and Adult Central Nervous System -- Cryosectioning -- The Golgi-Cox Method -- Neuroanatomical Tract-Tracing Using Biotinylated Dextran Amine -- Retrograde Tracing Technique for Neonatal Animals.
|
| 650 |
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0 |
|a Neurosciences.
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| 650 |
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0 |
|a Neurobiology.
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| 650 |
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0 |
|a Cell culture.
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| 650 |
1 |
4 |
|a Neurosciences.
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| 650 |
2 |
4 |
|a Neurobiology.
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| 650 |
2 |
4 |
|a Cell Culture.
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| 700 |
1 |
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|a Zhou, Renping.
|e editor.
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| 700 |
1 |
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|a Mei, Lin.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|