Neural Development : Methods and Protocols /
| Corporate Author: | |
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| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2013.
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| Edition: | 1st ed. 2013. |
| Series: | Methods in Molecular Biology,
1018 |
| Subjects: |
Table of Contents:
- Isolation and Maintenance of Cortical Neural Progenitor Cells In Vitro
- Neural Induction with a Dopaminergic Phenotype from Human Pluripotent Stem Cells Through a Feeder-Free Floating Aggregation Culture
- Nucleoside Analog Labeling of Neural Stem Cells and Their Progeny
- Culture of Dissociated Hippocampal Neurons
- Culturing Mouse Cerebellar Granule Neurons
- Preparation of Primary Cultured Dopaminergic Neurons from Mouse Brain
- Culturing Astoryctes from Postnatal Rats
- Culturing Oligodendrocyte Lineage Cells from Neonatal Rats
- Isolation and Culture of Schwann Cells
- DNA Transfection: Calcium Phosphate Method
- The Gene-Gun Approach for Transfection and Labeling of Cells in Brain Slices
- Lentiviral Vector Production, Titration, and Transduction of Primary Neurons
- In-Ovo Electroporation in Embryonic Chick Spinal Cords
- Gene Transfer in Developing Chick Embryos: In Ovo Electroporation
- In Utero Electroporation in Mice
- General Introduction to In Situ Hybridization Protocol Using Non-Radioactively Labeled Probes to Detect mRNAs on Tissue Sections
- Immunofluorescence Staining with Frozen Mouse or Chick Embryonic Tissue Sections
- β-Galactosidase Staining of LacZ Fusion Proteins in Whole Tissue Preparations
- Chromatin Immunoprecipitation Assay of Brain Tissues Using Percoll Gradient-Purified Nuclei
- In Vivo Dual Luciferase Reporter Assay with Chick Neural Tube In Ovo Electroporation System
- Growth Cone Collapse Assay
- The Stripe Assay: Studying Growth Preference and Axon Guidance on Binary Choice Substrates In Vitro
- Microcontact Printing of Substrate-Bound Protein Patterns for Cell- and Tissue Culture
- Semi-Automated Analysis of Dendrite Morphology in Cell Culture
- Monitoring Synaptic Plasticity by Imaging AMPA Receptor Content and Dynamics on Dendritic Spines
- Morphological Analysis of Neuromuscular Junctions by Immunofluorescent Staining of Whole-Mount Mouse Diaphragms- Routine Histology Techniques for the Developing and Adult Central Nervous System
- Cryosectioning
- The Golgi-Cox Method
- Neuroanatomical Tract-Tracing Using Biotinylated Dextran Amine
- Retrograde Tracing Technique for Neonatal Animals.