Protein Downstream Processing : Design, Development and Application of High and Low-Resolution Methods /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Labrou, Nikolaos E. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1129
Materias:
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020 |a 9781627039772 
024 7 |a 10.1007/978-1-62703-977-2  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a Protein Downstream Processing :  |b Design, Development and Application of High and Low-Resolution Methods /  |c edited by Nikolaos E. Labrou. 
250 |a 1st ed. 2014. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2014. 
300 |a XV, 555 p. 121 illus., 62 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 1129 
505 0 |a Protein Purification: An Overview -- Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoE -- High Throughput Process Development: I. Process Chromatography -- High Throughput Process Development: II. Membrane Chromatography -- Media Selection in Ion Exchange Chromatography in a Single Microplate -- High Throughput Screening of Dye-Ligands for Chromatography -- Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette Tips -- Recovery of Recombinant Proteins from Plants Using Aqueous Two-Phase Partitioning Systems: An Outline -- Aqueous Two-Phase Systems Strategies for the Recovery of Proteins from Plants -- Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins -- Simultaneous Purification and Refolding of Proteins by Affinity Precipitation and Macro-(Affinity Ligand) Facilitated Three Phase Partitioning (MLFTPP) -- Co-Expression and Co-Purification of Antigen-Antibody Complexes in Bacterial Cytoplasm and Periplasm -- Immunoglobulin Purification by Caprylic Acid -- Affinity Tags in Protein Purification and Peptide Enrichment: An Overview -- Expression and Purification of Large Active GST Fusion Enzymes -- Synthetic Ligand Affinity Chromatography. Purification of Human Serum Albumin and Related Fusion Proteins -- Zbasic - A Purification Tag for Selective Ion-Exchange Recovery -- An Orthogonal Fusion Tag for Efficient Protein Purification -- Phage Display of Engineered Binding Proteins -- Biomimetic Affinity Ligands for Protein Purification -- Synthesis and Application of Dye-Ligand Affinity Adsorbents -- Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification -- Affinity Chromatography of Proteins on Monolithic Columns -- Sample Displacement Batch Chromatography of Proteins -- Analysis of Host-Cell Proteins in Biotherapeutic Proteins by LC/MS Approaches -- Preparation of Monolithic Affinity Media for Nano-Liquid Chromatography Applications -- Proteomic Analysis of Complex Protein Samples by MALDI TOF Mass Spectrometry -- Modern Bioanalysis of Proteins by Electrophoretic Techniques -- Protein Structure Validation and Analysis with X-Ray Crystallography -- Measuring Binding Constants of His-Tagged Proteins using Affinity Chromatography and Ni-NTA Immobilized Enzymes -- Stabilization of Therapeutic Proteins in Aqueous Solutions and Freeze-Dried Solids: An Overview -- Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin -- G Protein-Coupled Receptor Expression and Purification.-  (Hyper)thermophilic Enzymes: Production and Purification -- Affinity Chromatography for Antibody Purification -- Screening and Purification of Recombinant Lignocellulolytic Enzymes -- Purification of PEGylated Proteins, with the Example of PEGylated lysozyme and PEGylated scFv -- One-Step Purification of Glutamate decarboxylase from E. coli Using Aqueous Two Phase System. 
650 0 |a Proteins . 
650 1 4 |a Protein Science. 
700 1 |a Labrou, Nikolaos E.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
900 |a Libro descargado a ALEPH en bloque (proveniente de proveedor)