Protein Downstream Processing : Design, Development and Application of High and Low-Resolution Methods /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Labrou, Nikolaos E. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1129
Materias:
Tabla de Contenidos:
  • Protein Purification: An Overview
  • Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoE
  • High Throughput Process Development: I. Process Chromatography
  • High Throughput Process Development: II. Membrane Chromatography
  • Media Selection in Ion Exchange Chromatography in a Single Microplate
  • High Throughput Screening of Dye-Ligands for Chromatography
  • Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette Tips
  • Recovery of Recombinant Proteins from Plants Using Aqueous Two-Phase Partitioning Systems: An Outline
  • Aqueous Two-Phase Systems Strategies for the Recovery of Proteins from Plants
  • Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins
  • Simultaneous Purification and Refolding of Proteins by Affinity Precipitation and Macro-(Affinity Ligand) Facilitated Three Phase Partitioning (MLFTPP)
  • Co-Expression and Co-Purification of Antigen-Antibody Complexes in Bacterial Cytoplasm and Periplasm
  • Immunoglobulin Purification by Caprylic Acid
  • Affinity Tags in Protein Purification and Peptide Enrichment: An Overview
  • Expression and Purification of Large Active GST Fusion Enzymes
  • Synthetic Ligand Affinity Chromatography. Purification of Human Serum Albumin and Related Fusion Proteins
  • Zbasic - A Purification Tag for Selective Ion-Exchange Recovery
  • An Orthogonal Fusion Tag for Efficient Protein Purification
  • Phage Display of Engineered Binding Proteins
  • Biomimetic Affinity Ligands for Protein Purification
  • Synthesis and Application of Dye-Ligand Affinity Adsorbents
  • Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification
  • Affinity Chromatography of Proteins on Monolithic Columns
  • Sample Displacement Batch Chromatography of Proteins
  • Analysis of Host-Cell Proteins in Biotherapeutic Proteins by LC/MS Approaches
  • Preparation of Monolithic Affinity Media for Nano-Liquid Chromatography Applications
  • Proteomic Analysis of Complex Protein Samples by MALDI TOF Mass Spectrometry
  • Modern Bioanalysis of Proteins by Electrophoretic Techniques
  • Protein Structure Validation and Analysis with X-Ray Crystallography
  • Measuring Binding Constants of His-Tagged Proteins using Affinity Chromatography and Ni-NTA Immobilized Enzymes
  • Stabilization of Therapeutic Proteins in Aqueous Solutions and Freeze-Dried Solids: An Overview
  • Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin
  • G Protein-Coupled Receptor Expression and Purification.-  (Hyper)thermophilic Enzymes: Production and Purification
  • Affinity Chromatography for Antibody Purification
  • Screening and Purification of Recombinant Lignocellulolytic Enzymes
  • Purification of PEGylated Proteins, with the Example of PEGylated lysozyme and PEGylated scFv
  • One-Step Purification of Glutamate decarboxylase from E. coli Using Aqueous Two Phase System.