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02687nam a22003135i 4500 |
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20210531091905.0 |
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cr nn 008mamaa |
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140607s2014 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493909223
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| 024 |
7 |
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|a 10.1007/978-1-4939-0922-3
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Plant MAP Kinases :
|b Methods and Protocols /
|c edited by George Komis, Jozef Šamaj.
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| 250 |
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|a 1st ed. 2014.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2014.
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| 300 |
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|a XI, 266 p. 33 illus., 17 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1171
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| 505 |
0 |
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|a Transient Expression in Arabidopsis Leaf Mesophyll Protoplast System for Cell-based Functional Analysis of MAPK Cascades Signaling -- Quantification of Stress Induced Mitogen-activated Protein Kinase Expressional Dynamic using Quantitative Real-time PCR -- Analysis of MAPK Activities using MAPK-specific Antibodies -- Detection of Protein Phosphorylation and Charge Isoforms using Vertical one-Dimensional Isoelectric Focusing Gels -- Affinity-based SDS PAGE Identification of Phosphorylated Arabidopsis MAPKs and Substrates by Acrylamide Pendant Phos-Tag™ -- Identification of Constitutively Active AtMPK6 Mutants using a Functional Screen in Saccharomyces Cerevisiae -- Virus-induced Gene Silencing in Plant MAPK Research -- RNA Interference of Plant MAPK Cascades for Functional Studies -- Immunofluorescent Localization of MAPKs and Colocalization with Microtubules in Arabidopsis Seedling Whole Mount Probes -- Immunofluorescent Localization of MAPKs in Steedman’s Wax Sections -- Fluorescent Protein Tagging of Arabidopsis MAPKs for In Vivo Localization Studies -- Bimolecular Fluorescent Complementation (BiFC) by MAP Kinases and MAPK Phosphatases -- Determination of Phosphorylation Sites in Microtubule Associated Protein MAP65-1 -- In vivo Phosphorylation of WRKY Transcription Factor by MAPK -- Rapid Mutagenesis-based Analysis of Phosphorylation Sites in Mitogen-activated Protein Kinase Substrates -- Yeast Two-hybrid System for Dissecting the Rice MAPK Interactome -- Experimental and Analytical Approaches to Characterize Plant Kinases using Protein Microarrays.
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| 650 |
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|a Plant science.
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| 650 |
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|a Botany.
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| 650 |
1 |
4 |
|a Plant Sciences.
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| 700 |
1 |
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|a Komis, George.
|e editor.
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| 700 |
1 |
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|a Šamaj, Jozef.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|