Plant MAP Kinases : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Komis, George. (Editor ), Šamaj, Jozef. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1171
Materias:
Tabla de Contenidos:
  • Transient Expression in Arabidopsis Leaf Mesophyll Protoplast System for Cell-based Functional Analysis of MAPK Cascades Signaling
  • Quantification of Stress Induced Mitogen-activated Protein Kinase Expressional Dynamic using Quantitative Real-time PCR
  • Analysis of MAPK Activities using MAPK-specific Antibodies
  • Detection of Protein Phosphorylation and Charge Isoforms using Vertical one-Dimensional Isoelectric Focusing Gels
  • Affinity-based SDS PAGE Identification of Phosphorylated Arabidopsis MAPKs and Substrates by Acrylamide Pendant Phos-Tag™
  • Identification of Constitutively Active AtMPK6 Mutants using a Functional Screen in Saccharomyces Cerevisiae
  • Virus-induced Gene Silencing in Plant MAPK Research
  • RNA Interference of Plant MAPK Cascades for Functional Studies
  • Immunofluorescent Localization of MAPKs and Colocalization with Microtubules in Arabidopsis Seedling Whole Mount Probes
  • Immunofluorescent Localization of MAPKs in Steedman’s Wax Sections
  • Fluorescent Protein Tagging of Arabidopsis MAPKs for In Vivo Localization Studies
  • Bimolecular Fluorescent Complementation (BiFC) by MAP Kinases and MAPK Phosphatases
  • Determination of Phosphorylation Sites in Microtubule Associated Protein MAP65-1
  • In vivo Phosphorylation of WRKY Transcription Factor by MAPK
  • Rapid Mutagenesis-based Analysis of Phosphorylation Sites in Mitogen-activated Protein Kinase Substrates
  • Yeast Two-hybrid System for Dissecting the Rice MAPK Interactome
  • Experimental and Analytical Approaches to Characterize Plant Kinases using Protein Microarrays.