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150112s2015 xxu| s |||| 0|eng d |
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|a 9781493922147
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| 024 |
7 |
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|a 10.1007/978-1-4939-2214-7
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a RNA Remodeling Proteins :
|b Methods and Protocols /
|c edited by Marc Boudvillain.
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| 250 |
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|a 1st ed. 2015.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2015.
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| 300 |
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|a XIV, 470 p. 84 illus., 40 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1259
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| 505 |
0 |
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|a Key Points to Consider When Studying RNA Remodeling by Proteins -- Happy Birthday: 25 years of DEAD-box Proteins -- In vivo Cross-linking Followed by polyA Enrichment to Identify Yeast mRNA Binding Proteins -- Dynamics of the Spb4 Interactome Monitored by Affinity Purification -- Using EMOTE to Map the Exact 5'-ends of Processed RNA on a Transcriptome-wide Scale -- Cellular Localization of RNA Degradation and Processing Components in Escherichia coli -- Recombineering Applications for the Mutational Analysis of Bacterial RNA-binding Proteins and Their Sites of Action -- Determination of RNA Chaperone Activity Using an Escherichia coli Mutant -- Biochemical Characterization of G4 Quadruplex Telomerase RNA Unwinding by the RNA Helicase RHAU -- ATPase Site Configuration of the RNA Helicase DbpA Probed by ENDOR Spectroscopy -- Bioinformatics and Biochemical Methods to Study the Structural and Functional Elements of DEAD-box RNA Helicases -- Measuring Helicase Inhibition of the DEAD-box Protein Dbp2 by Yra1 -- A FRET-based, Continuous Assay for the Helicase Activity of DEAD-box Proteins -- A Fluorescence-Based Screening Assay for Identification of Hepatitis C Virus NS3 Helicase Inhibitors and Characterization of Their Inhibitory Mechanism -- Mechanisms of HCV NS3 Helicase Monitored by Optical Tweezers -- Constructing a Magnetic Tweezers to Monitor RNA Translocation at the Single-molecule Level -- Probing RNA Translocases with DNA -- Monitoring RNA Unwinding by the Transcription Termination Factor Rho From Mycobacterium Tuberculosis -- Characterization of the Mechanisms of Transcription Termination by the Helicase Sen1 -- Characterization of TRAP-mediated Regulation of the B. subtilis trp Operon Using in vitro Transcription and Transcriptional Reporter Fusions in vivo -- Single-molecule FRET Characterization of RNA Remodeling Induced by an Antitermination Protein -- Fluorescence Reporters for Hfq Oligomerization and RNA Annealing -- Fluorescence Anisotropy-based Salt-titration Approach to Characterize Protein-Nucleic Acid Interactions -- Probing Hfq:RNA Interactions with Hydroxyl Radical and RNase Footprinting -- Purification of Eukaryotic Exoribonucleases Following Heterologous Expression in Bacteria and Analysis of Their Biochemical Properties by in vitro Enzymatic Assays -- Tips and Tricks to Probe the RNA-degrading Activities of Hyperthermophilic Archaeal β-CASP Ribonucleases.
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| 650 |
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|a Proteins .
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| 650 |
1 |
4 |
|a Protein Science.
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| 650 |
2 |
4 |
|a Protein Structure.
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| 700 |
1 |
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|a Boudvillain, Marc.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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