RNA Remodeling Proteins : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Edition: | 1st ed. 2015. |
| Series: | Methods in Molecular Biology,
1259 |
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Table of Contents:
- Key Points to Consider When Studying RNA Remodeling by Proteins
- Happy Birthday: 25 years of DEAD-box Proteins
- In vivo Cross-linking Followed by polyA Enrichment to Identify Yeast mRNA Binding Proteins
- Dynamics of the Spb4 Interactome Monitored by Affinity Purification
- Using EMOTE to Map the Exact 5'-ends of Processed RNA on a Transcriptome-wide Scale
- Cellular Localization of RNA Degradation and Processing Components in Escherichia coli
- Recombineering Applications for the Mutational Analysis of Bacterial RNA-binding Proteins and Their Sites of Action
- Determination of RNA Chaperone Activity Using an Escherichia coli Mutant
- Biochemical Characterization of G4 Quadruplex Telomerase RNA Unwinding by the RNA Helicase RHAU
- ATPase Site Configuration of the RNA Helicase DbpA Probed by ENDOR Spectroscopy
- Bioinformatics and Biochemical Methods to Study the Structural and Functional Elements of DEAD-box RNA Helicases
- Measuring Helicase Inhibition of the DEAD-box Protein Dbp2 by Yra1
- A FRET-based, Continuous Assay for the Helicase Activity of DEAD-box Proteins
- A Fluorescence-Based Screening Assay for Identification of Hepatitis C Virus NS3 Helicase Inhibitors and Characterization of Their Inhibitory Mechanism
- Mechanisms of HCV NS3 Helicase Monitored by Optical Tweezers
- Constructing a Magnetic Tweezers to Monitor RNA Translocation at the Single-molecule Level
- Probing RNA Translocases with DNA
- Monitoring RNA Unwinding by the Transcription Termination Factor Rho From Mycobacterium Tuberculosis
- Characterization of the Mechanisms of Transcription Termination by the Helicase Sen1
- Characterization of TRAP-mediated Regulation of the B. subtilis trp Operon Using in vitro Transcription and Transcriptional Reporter Fusions in vivo
- Single-molecule FRET Characterization of RNA Remodeling Induced by an Antitermination Protein
- Fluorescence Reporters for Hfq Oligomerization and RNA Annealing
- Fluorescence Anisotropy-based Salt-titration Approach to Characterize Protein-Nucleic Acid Interactions
- Probing Hfq:RNA Interactions with Hydroxyl Radical and RNase Footprinting
- Purification of Eukaryotic Exoribonucleases Following Heterologous Expression in Bacteria and Analysis of Their Biochemical Properties by in vitro Enzymatic Assays
- Tips and Tricks to Probe the RNA-degrading Activities of Hyperthermophilic Archaeal β-CASP Ribonucleases.