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150323s2015 xxu| s |||| 0|eng d |
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|a 9781493925698
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|a 10.1007/978-1-4939-2569-8
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Rab GTPases :
|b Methods and Protocols /
|c edited by Guangpu Li.
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|a 1st ed. 2015.
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2015.
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|a XIV, 358 p. 82 illus., 54 illus. in color. :
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 1298
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|a Rab Family of GTPases -- Bioinformatic Approaches to Identifying and Classifying Rab Proteins -- Rab-NANOPS: FRET-Biosensors for Rab Membrane Nano clustering and Prenylation Detection in Mammalian Cells -- High Throughput Assay for Profiling the Substrate Specificity of Rab GTPase-Activating Proteins -- Measuring Rab GTPase-Activating Protein (GAP) Activity in Live Cells and Extracts -- Analysis of the Interactions Between Rab GTPases and Class V Myosins -- Assaying the Interaction of the Rab Guanine Nucleotide Exchange Protein Sec2 with the Upstream Rab, a Downstream Effector and a Phosphoinositide -- Kinetic Activation of Rab8 Guanine Nucleotide Exchange Factor Rabin8 by Rab11 -- Ypt1 and TRAPP Interactions: Optimization of Multi-Color Bimolecular Fluorescence Complementation in Yeast -- Identifying a Rab Effector on the Macro autophagy Pathway -- Functional Analysis of Rab27 and its Effector Slp-2a in Renal Epithelial Cells -- Small GTPases in Acrosomal Exocytosis -- Rab Antibody Characterization: Comparison of Rab14 Antibodies -- Selective Visualization of GLUT4 Storage Vesicles and Associated Rab Proteins Using IRAP-pHluorin -- 3D Time-Lapse Analysis of Rab11/FIP5 Complex: Spatiotemporal Dynamics During Apical Lumen Formation -- In Vitro and In Vivo Characterization of the Rab11-GAP Activity of Drosophila Evi5 -- Characterization of the Role Rab25 in Energy Metabolism and Cancer Using Extracellular Flux Analysis and Material Balance -- Measurement of Rab35 Activity by the GTP-Rab35 Trapper RBD35 -- Analysis of Connecdenn 1-3 (DENN1A-C) GEF Activity for Rab35 -- Assay of Rab17 and its Guanine Nucleotide Exchange Factor Rabex-5 in Dendrites of Hippocampal Neurons -- Methods for Analysis of AP-3/Rabin4’ in Regulation of Lysosome Distribution -- Determination of Rab5 Activity in the Cell by Effector Pull-Down Assay -- Identification of the Rab5 Binding Site in p110b - Assays for PI3Kb Binding to Rab5 -- Role of the Rab5 Guanine Nucleotide Exchange Factor, Rme-6, in the Regulation of Clathrin-Coated Vesicle Uncoating -- Differential Effects of Overexpression of Rab5 and Rab22 on Autophagy in PC12 Cells With or Without NGF -- Determining the Role of Rab7 in Constitutive and Ligand-Mediated Epidermal Growth Factor Receptor Endocytic Trafficking Using Single Cell Assays -- Visualizing Directional Rab7 and TrkA Co-Trafficking in Axons by pTIRF Microscopy -- Quantitative Bead-Based Flow Cytometry for Assaying Rab7 GTPase Interaction with the Rab-Interacting Lysosomal Protein (RILP) Effector Protein.
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|a Biochemistry.
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|a Cell membranes .
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|a Biochemistry, general.
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|a Membrane Biology.
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|a Li, Guangpu.
|e editor.
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|a SpringerLink (Online service)
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|t Springer eBooks
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