Rab GTPases : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Li, Guangpu. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2015.
Edición:1st ed. 2015.
Colección:Methods in Molecular Biology, 1298
Materias:
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024 7 |a 10.1007/978-1-4939-2569-8  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a Rab GTPases :  |b Methods and Protocols /  |c edited by Guangpu Li. 
250 |a 1st ed. 2015. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2015. 
300 |a XIV, 358 p. 82 illus., 54 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 1298 
505 0 |a Rab Family of GTPases -- Bioinformatic Approaches to Identifying and Classifying Rab Proteins -- Rab-NANOPS: FRET-Biosensors for Rab Membrane Nano clustering and Prenylation Detection in Mammalian Cells -- High Throughput Assay for Profiling the Substrate Specificity of Rab GTPase-Activating Proteins -- Measuring Rab GTPase-Activating Protein (GAP) Activity in Live Cells and Extracts -- Analysis of the Interactions Between Rab GTPases and Class V Myosins -- Assaying the Interaction of the Rab Guanine Nucleotide Exchange Protein Sec2 with the Upstream Rab, a Downstream Effector and a Phosphoinositide -- Kinetic Activation of Rab8 Guanine Nucleotide Exchange Factor Rabin8 by Rab11 -- Ypt1 and TRAPP Interactions: Optimization of Multi-Color Bimolecular Fluorescence Complementation in Yeast -- Identifying a Rab Effector on the Macro autophagy Pathway -- Functional Analysis of Rab27 and its Effector Slp-2a in Renal Epithelial Cells -- Small GTPases in Acrosomal Exocytosis -- Rab Antibody Characterization: Comparison of Rab14 Antibodies -- Selective Visualization of GLUT4 Storage Vesicles and Associated Rab Proteins Using IRAP-pHluorin -- 3D Time-Lapse Analysis of Rab11/FIP5 Complex: Spatiotemporal Dynamics During Apical Lumen Formation -- In Vitro and In Vivo Characterization of the Rab11-GAP Activity of Drosophila Evi5 -- Characterization of the Role Rab25 in Energy Metabolism and Cancer Using Extracellular Flux Analysis and Material Balance -- Measurement of Rab35 Activity by the GTP-Rab35 Trapper RBD35 -- Analysis of Connecdenn 1-3 (DENN1A-C) GEF Activity for Rab35 -- Assay of Rab17 and its Guanine Nucleotide Exchange Factor Rabex-5 in Dendrites of Hippocampal Neurons -- Methods for Analysis of AP-3/Rabin4’ in Regulation of Lysosome Distribution -- Determination of Rab5 Activity in the Cell by Effector Pull-Down Assay -- Identification of the Rab5 Binding Site in p110b - Assays for PI3Kb Binding to Rab5 -- Role of the Rab5 Guanine Nucleotide Exchange Factor, Rme-6, in the Regulation of Clathrin-Coated Vesicle Uncoating -- Differential Effects of Overexpression of Rab5 and Rab22 on Autophagy in PC12 Cells With or Without NGF -- Determining the Role of Rab7 in Constitutive and Ligand-Mediated Epidermal Growth Factor Receptor Endocytic Trafficking Using Single Cell Assays -- Visualizing Directional Rab7 and TrkA Co-Trafficking in Axons by pTIRF Microscopy -- Quantitative Bead-Based Flow Cytometry for Assaying Rab7 GTPase Interaction with the Rab-Interacting Lysosomal Protein (RILP) Effector Protein. 
650 0 |a Biochemistry. 
650 0 |a Cell membranes . 
650 1 4 |a Biochemistry, general. 
650 2 4 |a Membrane Biology. 
700 1 |a Li, Guangpu.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks