Rab GTPases : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Li, Guangpu. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2015.
Edición:1st ed. 2015.
Colección:Methods in Molecular Biology, 1298
Materias:
Tabla de Contenidos:
  • Rab Family of GTPases
  • Bioinformatic Approaches to Identifying and Classifying Rab Proteins
  • Rab-NANOPS: FRET-Biosensors for Rab Membrane Nano clustering and Prenylation Detection in Mammalian Cells
  • High Throughput Assay for Profiling the Substrate Specificity of Rab GTPase-Activating Proteins
  • Measuring Rab GTPase-Activating Protein (GAP) Activity in Live Cells and Extracts
  • Analysis of the Interactions Between Rab GTPases and Class V Myosins
  • Assaying the Interaction of the Rab Guanine Nucleotide Exchange Protein Sec2 with the Upstream Rab, a Downstream Effector and a Phosphoinositide
  • Kinetic Activation of Rab8 Guanine Nucleotide Exchange Factor Rabin8 by Rab11
  • Ypt1 and TRAPP Interactions: Optimization of Multi-Color Bimolecular Fluorescence Complementation in Yeast
  • Identifying a Rab Effector on the Macro autophagy Pathway
  • Functional Analysis of Rab27 and its Effector Slp-2a in Renal Epithelial Cells
  • Small GTPases in Acrosomal Exocytosis
  • Rab Antibody Characterization: Comparison of Rab14 Antibodies
  • Selective Visualization of GLUT4 Storage Vesicles and Associated Rab Proteins Using IRAP-pHluorin
  • 3D Time-Lapse Analysis of Rab11/FIP5 Complex: Spatiotemporal Dynamics During Apical Lumen Formation
  • In Vitro and In Vivo Characterization of the Rab11-GAP Activity of Drosophila Evi5
  • Characterization of the Role Rab25 in Energy Metabolism and Cancer Using Extracellular Flux Analysis and Material Balance
  • Measurement of Rab35 Activity by the GTP-Rab35 Trapper RBD35
  • Analysis of Connecdenn 1-3 (DENN1A-C) GEF Activity for Rab35
  • Assay of Rab17 and its Guanine Nucleotide Exchange Factor Rabex-5 in Dendrites of Hippocampal Neurons
  • Methods for Analysis of AP-3/Rabin4’ in Regulation of Lysosome Distribution
  • Determination of Rab5 Activity in the Cell by Effector Pull-Down Assay
  • Identification of the Rab5 Binding Site in p110b - Assays for PI3Kb Binding to Rab5
  • Role of the Rab5 Guanine Nucleotide Exchange Factor, Rme-6, in the Regulation of Clathrin-Coated Vesicle Uncoating
  • Differential Effects of Overexpression of Rab5 and Rab22 on Autophagy in PC12 Cells With or Without NGF
  • Determining the Role of Rab7 in Constitutive and Ligand-Mediated Epidermal Growth Factor Receptor Endocytic Trafficking Using Single Cell Assays
  • Visualizing Directional Rab7 and TrkA Co-Trafficking in Axons by pTIRF Microscopy
  • Quantitative Bead-Based Flow Cytometry for Assaying Rab7 GTPase Interaction with the Rab-Interacting Lysosomal Protein (RILP) Effector Protein.