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150818s2015 xxu| s |||| 0|eng d |
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|a 9781493929351
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| 024 |
7 |
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|a 10.1007/978-1-4939-2935-1
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Protein Arginylation :
|b Methods and Protocols /
|c edited by Anna S. Kashina.
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| 250 |
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|a 1st ed. 2015.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2015.
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| 300 |
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|a IX, 149 p. 19 illus., 11 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1337
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|a Protein Arginylation: Over 50 Years of Discovery -- Recollection of How We Came Across the Protein Modification with Amino Acids by Aminoacyl tRNA-Protein Transferase -- Arginyltransferase: A Personal and Historical Perspective -- Arginylation in a Partially Purified Fraction of 150k xg Supernatants of Axoplasm and Injured Vertebrate Nerves -- Preparation of ATE1 Enzyme from Native Mammalian Tissues -- Correlated Measurement of Endogenous ATE1 Activity on Native Acceptor Proteins in Tissues and Cultured Cells to Detect Cellular Aging -- Assaying the Post-Translational Arginylation of Proteins in Cultured Cells -- Assaying ATE1 Activity in Yeast by β-gal Degradation -- Bacterial Expression and Purification of Recombinant Arginyltransferase (ATE1) and Arg-tRNA Synthetase (RRS) for Arginylation Assays -- Assaying ATE1 Activity In Vitro -- High Throughput Arginylation Assay in Micro plate Format -- Assay of Arginyltransferase Activity by a Fluorescent HPLC Method -- Identification of Arginylated Proteins by Mass Spectrometry -- Analysis of Arginylated Peptides by Subtractive Edman Degradation -- Transferase-Mediated Labeling of Protein N-Termini with Click Chemistry Handles -- Applying Arginylation for Bottom-Up Proteomics -- Development of New Tools for the Studies of Protein Arginylation.
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|a Proteins .
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| 650 |
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|a Protein Science.
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| 700 |
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|a Kashina, Anna S.
|e editor.
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| 710 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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