Protein Arginylation : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Edición: | 1st ed. 2015. |
| Colección: | Methods in Molecular Biology,
1337 |
| Materias: |
Tabla de Contenidos:
- Protein Arginylation: Over 50 Years of Discovery
- Recollection of How We Came Across the Protein Modification with Amino Acids by Aminoacyl tRNA-Protein Transferase
- Arginyltransferase: A Personal and Historical Perspective
- Arginylation in a Partially Purified Fraction of 150k xg Supernatants of Axoplasm and Injured Vertebrate Nerves
- Preparation of ATE1 Enzyme from Native Mammalian Tissues
- Correlated Measurement of Endogenous ATE1 Activity on Native Acceptor Proteins in Tissues and Cultured Cells to Detect Cellular Aging
- Assaying the Post-Translational Arginylation of Proteins in Cultured Cells
- Assaying ATE1 Activity in Yeast by β-gal Degradation
- Bacterial Expression and Purification of Recombinant Arginyltransferase (ATE1) and Arg-tRNA Synthetase (RRS) for Arginylation Assays
- Assaying ATE1 Activity In Vitro
- High Throughput Arginylation Assay in Micro plate Format
- Assay of Arginyltransferase Activity by a Fluorescent HPLC Method
- Identification of Arginylated Proteins by Mass Spectrometry
- Analysis of Arginylated Peptides by Subtractive Edman Degradation
- Transferase-Mediated Labeling of Protein N-Termini with Click Chemistry Handles
- Applying Arginylation for Bottom-Up Proteomics
- Development of New Tools for the Studies of Protein Arginylation.