Protein Chromatography : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Walls, Dermot. (Editor ), Loughran, Sinéad T. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:2nd ed. 2017.
Colección:Methods in Molecular Biology, 1485
Materias:
Tabla de Contenidos:
  • A Synopsis of Protein and their Purification
  • Gel-Filtration Chromatography
  • Immunoaffinity Chromatography: Concepts and Applications
  • Avoiding Proteolysis During Protein Purification
  • Scale-Up of Protein Purification: Downstream Processing Issues
  • Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources
  • Protein Stability: Enhancement and Measurement
  • Tagging Recombinant Proteins to Enhance Solubility and Aid Purification
  • Storage and Lyophilisation of Pure Proteins
  • Differential Precipitation and Solubilization of Proteins
  • Ion-Exchange Chromatography: Basic Principles and Application
  • Protein Quantitation and Analysis of Purity
  • Purification of Proteins Fused to Maltose-Binding Protein
  • Purification of Poly-Histidine-Tagged Proteins
  • Purification of Antibodies Using Affinity Chromatography
  • Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens
  • Measuring Protein-Protein Interactions Using Biacore
  • Hydrophobic Interaction Chromatography. .