Polycomb Group Proteins : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Lanzuolo, Chiara. (Editor ), Bodega, Beatrice. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1480
Materias:
Tabla de Contenidos:
  • Mapping the Function of Polycomb Proteins
  • Chromatin Immunoprecipitation
  • Chromatin preparation and Chromatin Immuno-Precipitation from Drosophila embryos
  • ChIP-seq data processing for PcG proteins and associated histone modifications
  • Analysis of single locus replication timing in asynchronous cycling cells
  • Non coding RNA interplay with the genome
  • RIP: RNA Immunoprecipitation
  • Capture Hybridization Analysis of DNA Targets
  • Identification of RNA-protein interactions through in-vitro RNA pull-down assays
  • Understanding RNA-chromatin interactions using Chromatin Isolation by RNA Purification (ChIRP)
  • Analysis RNA-seq and non coding RNA
  • The dynamics of Polycomb complexes
  • Fluorescence resonance energy transfer microscopy for measuring chromatin complex structure and dynamics
  • Analysis of endogenous protein interactions of Polycomb-group of proteins in mouse embryonic stem cells
  • Determination of Polycomb group of proteins compartmentalization through chromatin fractionation procedure
  • An Automatic Segmentation Method Combining an Active Contour Model and a Classification Technique for Detecting Polycomb-group Proteins in High-Throughput Microscopy Images
  • Polymer physics of the large-scale structure of chromatin
  • Chromosome Conformation Capture in Drosophila
  • Chromosome Conformation Capture in primary human cells
  • Determination of high-resolution 3D chromatin organization using Circular Chromosome Conformation Capture (4C-seq)
  • Chromosome Conformation Capture on Chip (4C): Data Processing
  • In vivo models to address the function of Polycomb Group Proteins
  • A rapid TALEN assembly protocol
  • Following the motion of Polycomb bodies in living Drosophila embryos
  • Reprogramming of Somatic Cells towards Pluripotency by Cell Fusion
  • Imaginal discs transplantation in Drosophila
  • Isolation and culture of muscle stem cells. .