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100623s2010 xxu| s |||| 0|eng d |
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|a 9781607617235
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|a 10.1007/978-1-60761-723-5
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Plant Secondary Metabolism Engineering :
|b Methods and Applications /
|c edited by Arthur Germano Fett-Neto.
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|a 1st ed. 2010.
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2010.
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|a X, 326 p. :
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 643
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|a Plant Secondary Metabolism and Challenges in Modifying Its Operation: An Overview -- Suppression Subtractive Hybridization as a Tool to Identify Anthocyanin Metabolism-Related Genes in Apple Skin -- Identification of Regulatory Protein Genes Involved in Alkaloid Biosynthesis Using a Transient RNAi System -- Site-Directed Mutagenesis and Saturation Mutagenesis for the Functional Study of Transcription Factors Involved in Plant Secondary Metabolite Biosynthesis -- Isolation of Proteins Binding to Promoter Elements of Alkaloid Metabolism-Related Genes Using Yeast One-Hybrid -- Modulation of Carotenoid Accumulation in Transgenic Potato by Inducing Chromoplast Formation with Enhanced Sink Strength -- Over-expression of Rate-Limiting Enzymes to Improve Alkaloid Productivity -- Microbial Expression of Alkaloid Biosynthetic Enzymes for Characterization of Their Properties -- Producing a Recombinant Flavin-Containing Monooxygenase from Coffea arabica in Escherichia coli for Screening of Potential Natural Substrates -- Efficient Production of Active Form Recombinant Cassava Hydroxynitrile Lyase Using Escherichia coli in Low-Temperature Culture -- of the Early Pathway to Taxol Biosynthesis in Yeast by Means of Biosynthetic Gene Cluster Construction Using SOE-PCR and Homologous Recombination -- Biocatalytic Synthesis of Tritium (3H)-Labelled Taxa-4(5),11(12)-diene, the Pathway Committing Precursor of the Taxoid Diterpenoids -- USER Cloning and USER Fusion: The Ideal Cloning Techniques for Small and Big Laboratories -- Enrichment of Carotenoids in Flaxseed by Introducing a Bacterial Phytoene Synthase Gene -- Metabolic Engineering by Plastid Transformation as a Strategy to Modulate Isoprenoid Yield in Plants -- Engineering High Yields of Secondary Metabolites in Rubia Cell Cultures Through Transformation with Rol Genes -- Flow Cytometric Methods to Investigate Culture Heterogeneities for Plant Metabolic Engineering -- Phenylpropanoid Biosynthesis in Leaves and Glandular Trichomes of Basil (Ocimum basilicum L.) -- Fusion with Fluorescent Proteins for Subcellular Localization of Enzymes Involved in Plant Alkaloid Biosynthesis -- Immunohistochemical Localisation of a Putative Flavonoid Transporter in Grape Berries -- Electrogenic Bromosulfalein Transport in Isolated Membrane Vesicles: Implementation in Both Animal and Plant Preparations for the Study of Flavonoid Transporters.
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|a Biotechnology.
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|a Plant science.
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|a Botany.
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|a Plant genetics.
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|a Gene expression.
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|a Biotechnology.
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|a Plant Sciences.
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|a Plant Genetics and Genomics.
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|a Gene Expression.
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|a Fett-Neto, Arthur Germano.
|e editor.
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|a SpringerLink (Online service)
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|t Springer eBooks
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