Plant Secondary Metabolism Engineering : Methods and Applications /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Fett-Neto, Arthur Germano. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2010.
Edición:1st ed. 2010.
Colección:Methods in Molecular Biology, 643
Materias:
Tabla de Contenidos:
  • Plant Secondary Metabolism and Challenges in Modifying Its Operation: An Overview
  • Suppression Subtractive Hybridization as a Tool to Identify Anthocyanin Metabolism-Related Genes in Apple Skin
  • Identification of Regulatory Protein Genes Involved in Alkaloid Biosynthesis Using a Transient RNAi System
  • Site-Directed Mutagenesis and Saturation Mutagenesis for the Functional Study of Transcription Factors Involved in Plant Secondary Metabolite Biosynthesis
  • Isolation of Proteins Binding to Promoter Elements of Alkaloid Metabolism-Related Genes Using Yeast One-Hybrid
  • Modulation of Carotenoid Accumulation in Transgenic Potato by Inducing Chromoplast Formation with Enhanced Sink Strength
  • Over-expression of Rate-Limiting Enzymes to Improve Alkaloid Productivity
  • Microbial Expression of Alkaloid Biosynthetic Enzymes for Characterization of Their Properties
  • Producing a Recombinant Flavin-Containing Monooxygenase from Coffea arabica in Escherichia coli for Screening of Potential Natural Substrates
  • Efficient Production of Active Form Recombinant Cassava Hydroxynitrile Lyase Using Escherichia coli in Low-Temperature Culture
  • of the Early Pathway to Taxol Biosynthesis in Yeast by Means of Biosynthetic Gene Cluster Construction Using SOE-PCR and Homologous Recombination
  • Biocatalytic Synthesis of Tritium (3H)-Labelled Taxa-4(5),11(12)-diene, the Pathway Committing Precursor of the Taxoid Diterpenoids
  • USER Cloning and USER Fusion: The Ideal Cloning Techniques for Small and Big Laboratories
  • Enrichment of Carotenoids in Flaxseed by Introducing a Bacterial Phytoene Synthase Gene
  • Metabolic Engineering by Plastid Transformation as a Strategy to Modulate Isoprenoid Yield in Plants
  • Engineering High Yields of Secondary Metabolites in Rubia Cell Cultures Through Transformation with Rol Genes
  • Flow Cytometric Methods to Investigate Culture Heterogeneities for Plant Metabolic Engineering
  • Phenylpropanoid Biosynthesis in Leaves and Glandular Trichomes of Basil (Ocimum basilicum L.)
  • Fusion with Fluorescent Proteins for Subcellular Localization of Enzymes Involved in Plant Alkaloid Biosynthesis
  • Immunohistochemical Localisation of a Putative Flavonoid Transporter in Grape Berries
  • Electrogenic Bromosulfalein Transport in Isolated Membrane Vesicles: Implementation in Both Animal and Plant Preparations for the Study of Flavonoid Transporters.