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03054nam a22003135i 4500 |
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20210907100654.0 |
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cr nn 008mamaa |
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150102s2015 xxu| s |||| 0|eng d |
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|a 9781493922536
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| 024 |
7 |
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|a 10.1007/978-1-4939-2253-6
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Nuclear Bodies and Noncoding RNAs :
|b Methods and Protocols /
|c edited by Shinichi Nakagawa, Tetsuro Hirose.
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| 250 |
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|a 1st ed. 2015.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2015.
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| 300 |
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|a XII, 351 p. 70 illus., 46 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1262
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| 505 |
0 |
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|a Visualization of lncRNA by Single Molecule Fluorescence in situ Hybridization -- Super-resolution Imaging of Nuclear Bodies by STED Microscopy -- High-resolution 3D DNA FISH Using Plasmid Probes and Computational Correction of Optical Aberrations to Study Chromatin Structure at the Sub-megabase Scale -- Time-lapse Imaging of Nuclear Bodies -- Visualization of Nucleic Acids with Synthetic Exciton-controlled Fluorescent Oligonucleotide Probes -- Live CLEM Imaging to Analyze Nuclear Structures at High-resolution -- Ultrastructural Analysis of Nuclear Bodies Using Electron Microscopy -- Analyses of Nuclear Proteins and Nucleic Acid Structures Using Atomic Force Microscopy -- Genome-wide Co-localization Screening of Nuclear Body Components Using a Fluorescently Tagged FLJ cDNA Clone Library -- Purification of Specific Chromatin Regions Using Oligonucleotides: Capture Hybridization Analysis of RNA Targets (CHART) -- RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin -- In situ Dissection of RNA Functional Subunits by Domain-specific Chromatin Isolation by RNA Purification (dChIRP) -- Extracting, Enriching and Identifying Nuclear Body Sub-complexes Using Label-based Quantitative Mass Spectrometry -- Studying RNA-binding Protein Interactions with Target mRNAs in Eukaryotic Cells: Native Ribonucleoprotein Immunoprecipitation (RIP) Assays -- Cross-linking and Immunoprecipitation of Nuclear RNA Binding Proteins -- Purifications of Non-coding RNA and Bound Proteins using FLAG Peptide-conjugated Antisense-oligonucleotides -- MMCT-Mediated Chromosome Engineering Technique Applicable to Functional Analysis of lncRNA and Nuclear Dynamics -- Reconstitution of Nucleocytoplasmic Transport Using Digitonin-Permeabilized Cells -- Genome-wide Analysis of Long Noncoding RNA Turnover -- Knockdown of Nuclear-retained Long Noncoding RNAs Using Modified DNA Antisense Oligonucleotides -- siRNA Screening of Nuclear Proteins.
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| 650 |
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|a Human genetics.
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| 650 |
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|a Medical genetics.
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| 650 |
1 |
4 |
|a Human Genetics.
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| 650 |
2 |
4 |
|a Gene Function.
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| 700 |
1 |
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|a Nakagawa, Shinichi.
|e editor.
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| 700 |
1 |
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|a Hirose, Tetsuro.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|