Nuclear Bodies and Noncoding RNAs : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Edición: | 1st ed. 2015. |
| Colección: | Methods in Molecular Biology,
1262 |
| Materias: |
Tabla de Contenidos:
- Visualization of lncRNA by Single Molecule Fluorescence in situ Hybridization
- Super-resolution Imaging of Nuclear Bodies by STED Microscopy
- High-resolution 3D DNA FISH Using Plasmid Probes and Computational Correction of Optical Aberrations to Study Chromatin Structure at the Sub-megabase Scale
- Time-lapse Imaging of Nuclear Bodies
- Visualization of Nucleic Acids with Synthetic Exciton-controlled Fluorescent Oligonucleotide Probes
- Live CLEM Imaging to Analyze Nuclear Structures at High-resolution
- Ultrastructural Analysis of Nuclear Bodies Using Electron Microscopy
- Analyses of Nuclear Proteins and Nucleic Acid Structures Using Atomic Force Microscopy
- Genome-wide Co-localization Screening of Nuclear Body Components Using a Fluorescently Tagged FLJ cDNA Clone Library
- Purification of Specific Chromatin Regions Using Oligonucleotides: Capture Hybridization Analysis of RNA Targets (CHART)
- RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin
- In situ Dissection of RNA Functional Subunits by Domain-specific Chromatin Isolation by RNA Purification (dChIRP)
- Extracting, Enriching and Identifying Nuclear Body Sub-complexes Using Label-based Quantitative Mass Spectrometry
- Studying RNA-binding Protein Interactions with Target mRNAs in Eukaryotic Cells: Native Ribonucleoprotein Immunoprecipitation (RIP) Assays
- Cross-linking and Immunoprecipitation of Nuclear RNA Binding Proteins
- Purifications of Non-coding RNA and Bound Proteins using FLAG Peptide-conjugated Antisense-oligonucleotides
- MMCT-Mediated Chromosome Engineering Technique Applicable to Functional Analysis of lncRNA and Nuclear Dynamics
- Reconstitution of Nucleocytoplasmic Transport Using Digitonin-Permeabilized Cells
- Genome-wide Analysis of Long Noncoding RNA Turnover
- Knockdown of Nuclear-retained Long Noncoding RNAs Using Modified DNA Antisense Oligonucleotides
- siRNA Screening of Nuclear Proteins.