PIWI-Interacting RNAs : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2014.
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| Edition: | 1st ed. 2014. |
| Series: | Methods in Molecular Biology,
1093 |
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Table of Contents:
- Chromatin Immunoprecipitation Assay of Piwi in Drosophila
- Drosophila Germline Stem Cell for in vitro Analyses of PIWI-Mediated RNAi
- RNAi and Overexpression of Genes in Ovarian Somatic Cells
- Making piRNAs in vitro
- A Framework for piRNA Cluster Manipulation
- Mass Spectrometric Analysis of 3'-End Methylation of piRNAs
- HITS-CLIP (CLIP-Seq) for Mouse Piwi Proteins
- Analysis of DNA Methylation in Mouse Testes
- Analysis of Small RNA-Guided Endonuclease Activity in Endogenous Piwi Protein Complexes from Mouse Testes
- Small RNA Library Construction from Minute Biological Samples
- Analysis of sDMA Modifications of PIWI Proteins
- Analyses of piRNA-Mediated Transcriptional Transposon Silencing in Drosophila: Nuclear Run-On Assay on Ovaries
- Combined RNA/DNA Fluorescence in situ Hybridization on Whole-Mount Drosophila Ovaries
- Fast and Accurate Method to Purify Small Non-Coding RNAs from Drosophila Ovaries
- Isolation of Zebrafish Gonads for RNA Isolation
- Small RNA Library Construction for High-Throughput Sequencing
- Analysis of Piwi-Loaded Small RNAs in Tetrahymena
- Effective Gene Knockdown in the Drosophila Germline by Artificial miRNA-Mimicking siRNAs
- Isolation and Bioinformatics Analyses of Small RNAs Interacting with Germ-Cell Specific Argonaute in Rice.