Nucleic Acid Crystallography : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Ennifar, Eric. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2016.
Edition:1st ed. 2016.
Series:Methods in Molecular Biology, 1320
Subjects:
Table of Contents:
  • Perspectives and Pitfalls in Nucleic Acids Crystallography
  • Preparation of Short 5'-Triphosphorylated Oligoribonucleotides for Crystallographic and Biochemical Studies
  • Preparation and Crystallization of Riboswitches
  • In Vitro/In Vivo Production of tRNA for X-Ray Studies
  • Polyacrylamide Gel Electrophoresis for Purification of Large Amounts of RNA
  • Use of the U1A Protein to Facilitate Crystallization and Structure Determination of Large RNAs
  • Fab Chaperone-Assisted RNA Crystallography (Fab CARC)
  • Generating Crystallographic Models of DNA Dodecamers from Structures of RNase H: DNA Complexes
  • Isothermal Titration Calorimetry: Assisted Crystallization of RNA-Ligand Complexes
  • Crystallographic Data and Model Quality
  • Advanced Crystallographic Data Collection Protocols for Experimental Phasing
  • Nucleic Acid Crystallography via Direct Selenium Derivatization: RNAs Modified with Se-Nucleobases
  • Practical Radiation Damage Induced Phasing
  • Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction Data
  • Using Molecular Replacement Phasing to Study the Structure and Function of RNA
  • Helical Symmetry of Nucleic Acids: Obstacle or Help in Structure Solution?
  • RNA Structure Refinement Using the ERRASER-Phenix Pipeline
  • Neutron Nucleic Acid Crystallography
  • Reconstitution of Functionally Active Thermus thermophilus 30S Ribosomal Subunit from Ribosomal 16S RNA and Ribosomal Proteins
  • Crystallographic Studies of the Ribosomal A-Site Molecular Switches by Using Model RNA Oligomers
  • Structure of the HCV Internal Ribosome Entry Site Subdomain IIa RNA in Complex with a Viral Translation Inhibitor
  • Anions in Nucleic Acid Crystallography.