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03489nam a22003495i 4500 |
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20220906161542.0 |
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cr nn 008mamaa |
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100301s2008 xxu| s |||| 0|eng d |
| 020 |
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|a 9781603274753
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| 024 |
7 |
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|a 10.1007/978-1-60327-475-3
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a RNA-Protein Interaction Protocols /
|c edited by Ren-Jang Lin.
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| 250 |
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|a 2nd ed. 2008.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2008.
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| 300 |
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|a XVI, 416 p. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 488
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| 505 |
0 |
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|a Isolation of a Sequence-Specific RNA Binding Protein, Polypyrimidine Tract Binding Protein, Using RNA Affinity Chromatography -- An Affinity Oligonucleotide Displacement Strategy to Purify Ribonucleoprotein Complexes Applied to Human Telomerase -- RNA Affinity Tags for the Rapid Purification and Investigation of RNAs and RNA–Protein Complexes -- Assembly and Glycerol Gradient Isolation of Yeast Spliceosomes Containing Transcribed or Synthetic U6 snRNA -- Purification of Ribonucleoproteins Using Peptide-Elutable Antibodies and Other Affinity Techniques -- CLIP: Crosslinking and ImmunoPrecipitation of In Vivo RNA Targets of RNA-Binding Proteins -- Quantitative Analysis of Protein-RNA Interactions by Gel Mobility Shift -- Monitoring Assembly of Ribonucleoprotein Complexes by Isothermal Titration Calorimetry -- Characterization of RNA—Protein Interactions by Phosphorothioate Footprinting and Its Applications to the Ribosome -- In Vivo Analysis of Ribonucleoprotein Complexes Using Nucleotide Analog Interference Mapping -- T7 RNA Polymerase-Mediated Incorporation of 8-N3AMP Into RNA for Studying Protein-RNA Interactions -- A Simple Crosslinking Method, CLAMP, to Map the Sites of RNA-Contacting Domains Within a Protein -- Proteins Specifically Modified With a Chemical Nuclease as Probes of RNA-Protein Interaction -- RNA-Protein Crosslink Mapping Using TEV Protease -- Structural Analysis of Protein-RNA Interactions With Mass Spectrometry -- Analyzing RNA-Protein Crosslinking Sites in Unlabeled Ribonucleoprotein Complexes by Mass Spectrometry -- In Vitro Selection of Random RNA Fragments to Identify Protein-Binding Sites Within Large RNAs -- Immunoprecipitation Analysis to Study RNA-Protein Interactions in Xenopus Oocytes -- Mapping the Regions of RNase P Catalytic RNA That Are Potentially in Close Contact With Its Protein Cofactor -- Quantification of MicroRNAs, Splicing Isoforms, and Homologous mRNAs With the Invader Assay -- Analysis of RNA Structure and RNA-Protein Interactions in Mammalian Cells by Use of Terminal Transferase-Dependent PCR -- Duplex Unwinding and RNP Remodeling With RNA Helicases -- Preparation of Efficient Splicing Extracts From Whole Cells, Nuclei, and Cytoplasmic Fractions -- Designing and Utilization of siRNAs Targeting RNA Binding Proteins -- The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
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0 |
|a Proteins .
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| 650 |
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0 |
|a Human genetics.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Protein Science.
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| 650 |
2 |
4 |
|a Human Genetics.
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| 700 |
1 |
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|a Lin, Ren-Jang.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|