RNA-Protein Interaction Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2008.
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| Edición: | 2nd ed. 2008. |
| Colección: | Methods in Molecular Biology,
488 |
| Materias: |
Tabla de Contenidos:
- Isolation of a Sequence-Specific RNA Binding Protein, Polypyrimidine Tract Binding Protein, Using RNA Affinity Chromatography
- An Affinity Oligonucleotide Displacement Strategy to Purify Ribonucleoprotein Complexes Applied to Human Telomerase
- RNA Affinity Tags for the Rapid Purification and Investigation of RNAs and RNA–Protein Complexes
- Assembly and Glycerol Gradient Isolation of Yeast Spliceosomes Containing Transcribed or Synthetic U6 snRNA
- Purification of Ribonucleoproteins Using Peptide-Elutable Antibodies and Other Affinity Techniques
- CLIP: Crosslinking and ImmunoPrecipitation of In Vivo RNA Targets of RNA-Binding Proteins
- Quantitative Analysis of Protein-RNA Interactions by Gel Mobility Shift
- Monitoring Assembly of Ribonucleoprotein Complexes by Isothermal Titration Calorimetry
- Characterization of RNA—Protein Interactions by Phosphorothioate Footprinting and Its Applications to the Ribosome
- In Vivo Analysis of Ribonucleoprotein Complexes Using Nucleotide Analog Interference Mapping
- T7 RNA Polymerase-Mediated Incorporation of 8-N3AMP Into RNA for Studying Protein-RNA Interactions
- A Simple Crosslinking Method, CLAMP, to Map the Sites of RNA-Contacting Domains Within a Protein
- Proteins Specifically Modified With a Chemical Nuclease as Probes of RNA-Protein Interaction
- RNA-Protein Crosslink Mapping Using TEV Protease
- Structural Analysis of Protein-RNA Interactions With Mass Spectrometry
- Analyzing RNA-Protein Crosslinking Sites in Unlabeled Ribonucleoprotein Complexes by Mass Spectrometry
- In Vitro Selection of Random RNA Fragments to Identify Protein-Binding Sites Within Large RNAs
- Immunoprecipitation Analysis to Study RNA-Protein Interactions in Xenopus Oocytes
- Mapping the Regions of RNase P Catalytic RNA That Are Potentially in Close Contact With Its Protein Cofactor
- Quantification of MicroRNAs, Splicing Isoforms, and Homologous mRNAs With the Invader Assay
- Analysis of RNA Structure and RNA-Protein Interactions in Mammalian Cells by Use of Terminal Transferase-Dependent PCR
- Duplex Unwinding and RNP Remodeling With RNA Helicases
- Preparation of Efficient Splicing Extracts From Whole Cells, Nuclei, and Cytoplasmic Fractions
- Designing and Utilization of siRNAs Targeting RNA Binding Proteins
- The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins.