Plant Fungal Pathogens : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Bolton, Melvin D. (Editor), Thomma, Bart P.H.J. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2012.
Edition:1st ed. 2012.
Series:Methods in Molecular Biology, 835
Subjects:
Table of Contents:
  • Fungal ABC Transporter Deletion and Localization Analysis
  • Targeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens Mediated Transformation
  • Activity-based Protein Profiling of Infected Plants
  • The use of Agroinfiltration for Transient Expression of Plant Resistance and Fungal Effector Proteins in Nicotiana benthamiana Leaves
  • Proteomic Techniques for Plant-Fungal Interactions
  • Identification of HR-inducing cDNAs from Plant Pathogens via a Gateway®-Compatible Binary Potato Virus X-Expression Vector
  • Freeze-Fracture of Infected Plant Leaves in Ethanol for Scanning Electron Microscopic Study of Fungal Pathogens
  • Real-time PCR for the Quantification of Fungi in planta
  • Using Non-homologous End-joining-deficient Strains for Functional Gene Analyses in Filamentous Fungi
  • Atomic Force Microscopy (AFM): A Tool for Studying Biophysical Surface Properties Underpinning Fungal Interactions with Plants and Substrates
  • Use of the Yeast Two-Hybrid System to Identify Targets of Fungal Effectors
  • Characterization of Plant - Fungal Interactions Involving Necrotrophic Effector-Producing Plant Pathogens
  • Heterologous Production of Fungal Effectors in Pichia pastoris
  • The Application of Laser Microdissection to Profiling Fungal Pathogen Gene Expression in planta
  • Metabolomics Protocols for Filamentous Fungi
  • Targeted Gene Replacement in Fungi Using a Split-Marker Approach
  • Massively Parallel Sequencing Technology in Pathogenic Microbes
  • Confocal Microscopy in Plant Pathogen Interactions
  • The use of Open Source Bioinformatics Tools to Dissect Transcriptomic Data
  • Population Biology of Fungal Plant Pathogens
  • Polyethylene Glycol (PEG)-mediated Transformation in Filamentous Fungal Pathogens
  • In vitro Induction of Infection-related Hyphal Structures in Plant Pathogenic Fungi
  • Fungicide Resistance Assays for Fungal Plant Pathogens
  • Identification of Lipid-binding Effectors
  • In silico Identification and Characterization of Effector Catalogs
  • Horizontal Transfer of Supernumerary Chromosomes in Fungi
  • The Induction of Mycotoxins by Trichothecene Producing Fusarium Species
  • Identifying Genes in Fusarium verticillioides Through Forward and Reverse Genetics
  • Assessment of Autophagosome Formation by Transmission Electron Microscopy (TEM)
  • Fungal Plant Pathogen Detection in Plant and Soil Samples using DNA Macroarrays
  • Random Insertional Mutagenesis in Fungal Genomes to Identify Virulence Factors
  • A Yeast-Secretion Trap Assay for Identification of Secreted Proteins from Eukaryotic Phytopathogens and their Plant Hosts
  • Comparing Fungal Genomes: Insight into Functional and Evolutionary Processes
  • Multi-gene Phylogenetic Analyses to Delimit New Species in Fungal Plant Pathogens
  • MAP Kinase Phosphorylation and cAMP Assessment in Fungi
  • A One-step Affinity-purification Protocol to Purify NB-LRR Immune Receptors from Plants that Mediate Resistance to Fungal Pathogens
  • Karyotyping Methods for Fungi
  • Isolation of Apoplastic Fluid from Leaf Tissue by the Vacuum Infiltration-Centrifugation Technique
  • Gene Cloning using Degenerate Primers and Genome Walking
  • Construction of Hairpin RNA Expressing Vectors for RNA-mediated Gene Silencing in Fungi
  • An Unbiased Method for the Quantitation of Disease Phenotypes using a Custom-built Macro Plugin for the Program ImageJ.