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03124nam a22003135i 4500 |
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20210602154650.0 |
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cr nn 008mamaa |
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101029s2011 xxu| s |||| 0|eng d |
| 020 |
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|a 9781607619130
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| 024 |
7 |
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|a 10.1007/978-1-60761-913-0
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Protein Chromatography :
|b Methods and Protocols /
|c edited by Dermot Walls, Sinéad T. Loughran.
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| 250 |
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|a 1st ed. 2011.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2011.
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| 300 |
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|a X, 527 p. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 681
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| 505 |
0 |
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|a A Digest of Protein Purification -- Gel-Filtration Chromatography -- Immunoaffinity Chromatography -- Avoiding Proteolysis During Protein Chromatography -- Scale-Up of Protein Purification: Downstream Processing Issues -- Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources -- Engineering Protein Stability -- Microfluidics in Protein Chromatography -- Tagging Recombinant Proteins to Enhance Solubility and Aid Purification -- Storage and Lyophilization of Pure Proteins -- Differential Precipitation and Solubilization of Proteins -- Ion-Exchange Chromatography: Basic Principles and Application to the Partial Purification of Soluble Mammalian Prolyl Oligopeptidase -- Protein Quantitation and Analysis of Purity -- Purification of Proteins Fused to Glutathione S-Tranferase -- Purification of Proteins Fused to Maltose-Binding Protein -- Purification of Proteins from Baculovirus-Infected Insect Cells -- Purification of Poly-Histidine-Tagged Proteins -- Immobilized Metal Affinity Chromatography/Reversed-Phase Enrichment of Phosphopeptides and Analysis by CID/ETD Tandem Mass Spectrometry -- Tag Removal by Site-Specific Cleavage of Recombinant Fusion Proteins -- Purification of Antibodies Using Affinity Chromatography -- Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens -- Measuring Protein-Protein Interactions Using Biacore -- Ultra-Performance Liquid Chromatography-Mass Spectrometry of Proteins -- Hydrophobic Interaction Chromatography -- Fast Protein Liquid Chromatography -- Shotgun Proteomics: A Qualitative Approach Applying Isoelectric Focusing on Immobilized pH Gradient and LC-MS/MS -- Shotgun Proteomics: A Relative Quantitative Approach Using Off-Gel Electrophoresis and LC-MS/MS -- Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC/MS) Purification Systems -- Strategies for the Purification of Membrane Proteins -- A Multi-Step Chromatographic Strategy to Purify Three Fungal Endo-β-Glucanases.
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| 650 |
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0 |
|a Proteins .
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| 650 |
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0 |
|a Chromatography.
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| 650 |
1 |
4 |
|a Protein Science.
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| 650 |
2 |
4 |
|a Chromatography.
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| 700 |
1 |
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|a Walls, Dermot.
|e editor.
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| 700 |
1 |
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|a Loughran, Sinéad T.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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