Protein Chromatography : Methods and Protocols /
| Autor Corporativo: | |
|---|---|
| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2011.
|
| Edición: | 1st ed. 2011. |
| Colección: | Methods in Molecular Biology,
681 |
| Materias: |
Tabla de Contenidos:
- A Digest of Protein Purification
- Gel-Filtration Chromatography
- Immunoaffinity Chromatography
- Avoiding Proteolysis During Protein Chromatography
- Scale-Up of Protein Purification: Downstream Processing Issues
- Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources
- Engineering Protein Stability
- Microfluidics in Protein Chromatography
- Tagging Recombinant Proteins to Enhance Solubility and Aid Purification
- Storage and Lyophilization of Pure Proteins
- Differential Precipitation and Solubilization of Proteins
- Ion-Exchange Chromatography: Basic Principles and Application to the Partial Purification of Soluble Mammalian Prolyl Oligopeptidase
- Protein Quantitation and Analysis of Purity
- Purification of Proteins Fused to Glutathione S-Tranferase
- Purification of Proteins Fused to Maltose-Binding Protein
- Purification of Proteins from Baculovirus-Infected Insect Cells
- Purification of Poly-Histidine-Tagged Proteins
- Immobilized Metal Affinity Chromatography/Reversed-Phase Enrichment of Phosphopeptides and Analysis by CID/ETD Tandem Mass Spectrometry
- Tag Removal by Site-Specific Cleavage of Recombinant Fusion Proteins
- Purification of Antibodies Using Affinity Chromatography
- Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens
- Measuring Protein-Protein Interactions Using Biacore
- Ultra-Performance Liquid Chromatography-Mass Spectrometry of Proteins
- Hydrophobic Interaction Chromatography
- Fast Protein Liquid Chromatography
- Shotgun Proteomics: A Qualitative Approach Applying Isoelectric Focusing on Immobilized pH Gradient and LC-MS/MS
- Shotgun Proteomics: A Relative Quantitative Approach Using Off-Gel Electrophoresis and LC-MS/MS
- Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC/MS) Purification Systems
- Strategies for the Purification of Membrane Proteins
- A Multi-Step Chromatographic Strategy to Purify Three Fungal Endo-β-Glucanases.