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140303s2014 xxu| s |||| 0|eng d |
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|a 9781627039710
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| 024 |
7 |
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|a 10.1007/978-1-62703-971-0
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Polyadenylation :
|b Methods and Protocols /
|c edited by Joanna Rorbach, Agnieszka J. Bobrowicz.
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| 250 |
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|a 1st ed. 2014.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2014.
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| 300 |
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|a XIV, 377 p. 63 illus., 22 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 1125
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| 505 |
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|a 3' end Formation and Regulation of Eukaryotic mRNAs -- Poly(A) Polymerase-based Poly(A) Length assay -- Using Klenow Mediated Extension to Measure Poly(A)-tail Length and Position in the Transcriptome -- Detection of Uridylated mRNAs -- Cytoplasmic Polyadenylation Assays -- In vitro Analysis of Transcriptional Activators and Polyadenylation -- Fluorescence Detection of Polyadenylation Reaction through the Coordination of Adensoine2-coralyne-adensoine2 -- Small molecule recognition of poly(A).-The Stem-loop Luciferase assay for Polyadenylation (SLAP) Method for Determining CstF-64-dependent Polyadenylation Activity -- Accurate Mapping of Cleavage and Polyadenylation Sites by 3' Region Extraction and Deep Sequencing -- Polyadenylation Site Prediction using PolyA-iEP Method -- Detecting alternative polyadenylation from Microarray Data -- Quantitative Polyadenylation Site Mapping with Single-molecule Direct RNA Sequencing -- RHAPA: A New Method to Quantify Alternative Polyadenylation -- Multiplex Analysis of PolyA-linked Sequences (MAPS): An RNA-seq Strategy to Profile poly(A+) RNA -- Global and Quantitative Profiling of Polyadenylated RNAs using PAS-seq -- High-throughput Sequencing of RNA Isolated by Crosslinking and Immunoprecipitation (HITS-CLIP) to Determine Sites of Binding of CstF-64 on Nascent RNAs -- Polyadenylation in Bacteria and Organelles -- In vivo Analysis of Polyadenylation in Prokaryotes -- Polyadenylation of RNA in E. coli: RNA Polymerase-associated (rA)n-synthetic Activities -- Analysis of the Human Mitochondrial Transcriptome using Directional Deep sequencing and Parallel Analysis of RNA Ends -- Measurement of Mitochondrial RNA Stability by Metabolic Labeling of Transcripts with 4-Thiouridine -- Deadenylation and its Regulation in Eukaryotic Cells -- Assaying mRNA Deadenylation in vitro -- Assaying mRNA Deadenylation in vivo -- In vitro Analysis of RNA Degradation Catalyzed by Deadenylase Enzymes -- microRNA-mediated Deadenylation in a Mammalian Cell-free System -- Quantitative Analysis of Deadenylation-independent mRNA Decay by a Modified MBRACE Assay.
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| 650 |
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|a Human genetics.
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| 650 |
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|a Medical genetics.
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| 650 |
1 |
4 |
|a Human Genetics.
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| 650 |
2 |
4 |
|a Gene Function.
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| 700 |
1 |
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|a Rorbach, Joanna.
|e editor.
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| 700 |
1 |
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|a Bobrowicz, Agnieszka J.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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