Polyadenylation : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Rorbach, Joanna. (Editor ), Bobrowicz, Agnieszka J. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1125
Materias:
Tabla de Contenidos:
  • 3’ end Formation and Regulation of Eukaryotic mRNAs
  • Poly(A) Polymerase-based Poly(A) Length assay
  • Using Klenow Mediated Extension to Measure Poly(A)-tail Length and Position in the Transcriptome
  • Detection of Uridylated mRNAs
  • Cytoplasmic Polyadenylation Assays
  • In vitro Analysis of Transcriptional Activators and Polyadenylation
  • Fluorescence Detection of Polyadenylation Reaction through the Coordination of Adensoine2-coralyne-adensoine2
  • Small molecule recognition of poly(A).-The Stem-loop Luciferase assay for Polyadenylation (SLAP) Method for Determining CstF-64-dependent Polyadenylation Activity
  • Accurate Mapping of Cleavage and Polyadenylation Sites by 3' Region Extraction and Deep Sequencing
  • Polyadenylation Site Prediction using PolyA-iEP Method
  • Detecting alternative polyadenylation from Microarray Data
  • Quantitative Polyadenylation Site Mapping with Single-molecule Direct RNA Sequencing
  • RHAPA: A New Method to Quantify Alternative Polyadenylation
  • Multiplex Analysis of PolyA-linked Sequences (MAPS): An RNA-seq Strategy to Profile poly(A+) RNA
  • Global and Quantitative Profiling of Polyadenylated RNAs using PAS-seq
  • High-throughput Sequencing of RNA Isolated by Crosslinking and Immunoprecipitation (HITS-CLIP) to Determine Sites of Binding of CstF-64 on Nascent RNAs
  • Polyadenylation in Bacteria and Organelles
  • In vivo Analysis of Polyadenylation in Prokaryotes
  • Polyadenylation of RNA in E. coli: RNA Polymerase-associated (rA)n-synthetic Activities
  • Analysis of the Human Mitochondrial Transcriptome using Directional Deep sequencing and Parallel Analysis of RNA Ends
  • Measurement of Mitochondrial RNA Stability by Metabolic Labeling of Transcripts with 4-Thiouridine
  • Deadenylation and its Regulation in Eukaryotic Cells
  • Assaying mRNA Deadenylation in vitro
  • Assaying mRNA Deadenylation in vivo
  • In vitro Analysis of RNA Degradation Catalyzed by Deadenylase Enzymes
  • microRNA-mediated Deadenylation in a Mammalian Cell-free System
  • Quantitative Analysis of Deadenylation-independent mRNA Decay by a Modified MBRACE Assay.